紫苏脂肪酸硫酯酶基因PfFatA生物信息学及其表达特性分析

发布时间：2019-03-15 17:23

【摘要】：为研究脂肪酸硫酯酶A(FatA)在紫苏脂肪酸合成机制中发挥的作用,对紫苏FatA基因及其编码的蛋白质进行生物信息学分析。结果表明,PfFatA基因cDNA全长1 652 bp,开放阅读框1 119 bp,编码372个氨基酸;紫苏FatA基因编码的蛋白质为亲水性不稳定蛋白,无跨膜区域;含Acyl-ACP_TE保守结构域,属于Hotdog fold超蛋白家族;多序列比对结果发现,序列C端含有保守的三联肽AKL和SKV结构;系统进化树分析表明,紫苏与丹参亲缘关系较近。通过Real-time PCR分析得知,PfFatA在紫苏根、茎、叶、花和种子不同发育时期均有表达,其中,在种子发育初期表达量最高。该研究结果可为进一步阐明PfFatA基因的功能及作用机制奠定基础。
[Abstract]:In order to study the role of fatty acid thioesterase A (FatA) in the fatty acid synthesis mechanism of Perilla perilla, bioinformatics analysis of perilla FatA gene and its encoded protein was carried out. The results showed that the full length cDNA of PfFatA gene was 1 652 bp, open reading frame (ORF) 1 119 bp, encoding 372 amino acids, the protein encoded by perilla FatA gene was a hydrophilic unstable protein with no transmembrane region. The results of multi-sequence alignment showed that the C-terminal of the sequence contained conserved tripeptide AKL and SKV, and phylogenetic tree analysis showed that the relationship between Radix Perilla and Salvia miltiorrhiza was close to that of Radix Perilla and Salvia miltiorrhiza (Salvia miltiorrhiza). Real-time PCR analysis showed that PfFatA was expressed in the root, stem, leaf, flower and seed of Perilla perilla at different developmental stages, and the highest expression was found in the early stage of seed development. The results of this study may lay a foundation for further elucidating the function and mechanism of PfFatA gene.
【作者单位】： 山西农业大学农学院;
【基金】：国家自然科学基金项目(31201266)
【分类号】：S565.9
，

本文编号：2440832