黄连素对2型糖尿病地鼠内脏脂肪组织RIP140调控通路基因mRNA表达的影响

发布时间：2019-03-15 21:17

【摘要】：目的:研究黄连素(BBR)对肥胖2型糖尿病(OT2DM)中国地鼠内脏白色脂肪组织(VWAT)中受体相互作用蛋白140(Receptor-interacting protein 140,RIP140)/PR结构域蛋白16(PR domain containing 16,PRDM16)信号通路基因mRNA表达的影响及相关机制。方法:以高脂饮食诱导肥胖胰岛素抵抗(OIR)地鼠模型,然后给予小剂量链脲菌素(STZ)建立OT2DM地鼠模型。造模完成后随机分成对照组、OIR组、OT2DM组和OT2DM BBR治疗组。BBR治疗9周,应用real-time RT-PCR技术检测各组地鼠VWAT中RIP140/PRDM16信号通路基因mRNA表达改变。结果:模型地鼠VWAT中RIP140、PKCε、PRMT1、Exportin1和白脂组织特异基因Resistin和Serpina3k的mRNA表达增加,而PRDM16、CtBP-1、CtBP-2、C/EBPβ、PPARγ、PGC-1α、PGC-1β、Gyk、GPDH、AQP7、GLUT4及棕脂组织特异基因UCP-1、Cidea、Elovl3和PPARα的mRNA表达降低。BBR治疗抑制VWAT中RIP140调控通路,诱导PRDM16信号通路,诱导棕脂组织特异基因mRNA的表达,抑制白色脂肪选择性基因的表达,诱导VWAT棕色化,改善脂诱性胰岛素抵抗(FIIR)。结论:RIP140/PRDM16信号通路参与BBR诱导VWAT棕色化的分子机制。
[Abstract]:Aim: to investigate the effect of berberine (BBR) on (VWAT) receptor-interacting protein 140 (Receptor-interacting protein 140, RP 140) / PR domain protein 16 (PR domain containing 16 in visceral white adipose tissue of Chinese hamsters with obese type 2 diabetes mellitus (OT2DM). The effect of PRDM16) signaling pathway gene mRNA expression and its related mechanism. Methods: (OIR) hamster model of obese insulin resistance was induced by high fat diet, and then OT2DM hamster model was established by low dose streptozotocin (STZ). After modeling, they were randomly divided into three groups: control group, OIR group, OT2DM group and OT2DM BBR treatment group. BBR was treated for 9 weeks. The expression of RIP140/PRDM16 signaling pathway gene mRNA in hamster VWAT was detected by real-time RT-PCR technique. Results: the expression of RIP140,PKC 蔚, PRMT1,Exportin1, Resistin and Serpina3k mRNA in model hamster VWAT was increased, while PRDM16,CtBP-1,CtBP-2,C/EBP 尾, PPAR 纬, PGC-1 伪, PGC-1 尾, Gyk,GPDH,AQP7, were increased. The mRNA expression of GLUT4 and brown fat tissue-specific genes UCP-1,Cidea,Elovl3 and PPAR 伪 decreased. BBR therapy inhibited the RIP140 regulation pathway in VWAT, induced PRDM16 signaling pathway, induced the expression of brown fat tissue-specific gene mRNA, and inhibited the expression of white fat selective gene. Induction of VWAT browning and improvement of lipid-induced insulin resistance (FIIR). Conclusion: RIP140/PRDM16 signaling pathway is involved in the molecular mechanism of VWAT browning induced by BBR.
【作者单位】：大连医科大学附属第一医院病理科;大连医科大学附属大连市中心医院内分泌科;
【分类号】：R285.5

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