不同鸡种BF2基因序列比较分析

发布时间：2019-03-18 19:25

【摘要】：利用目标区域捕获测序技术对BF2(Major histocompatibility complex class I antigen BF2)基因进行序列测定,找出不同鸡种BF2基因序列上的差异。以NCBI网站上Gallus_gallus-5.0的BF2基因序列为参照标准。利用MEGA6.06软件对25个鸡种的基因序列进行比对分析,统计SNPs位点和Indel位点,并构建系统进化树进行分析。结果表明:在BF2第2、3外显子上分别存在22和31个有义突变,占BF2基因全部有义突变的78%,表明BF2基因的第2、3外显子起重要作用且拥有丰富的多态性。第5、6、7外显子属于保守区域。25个鸡种大致可被分为3大类:鹿苑鸡、如皋黄鸡、溧阳鸡和太湖鸡等聚为一类;罗斯鸡和隐性白羽肉鸡等聚为一类;雪山鸡和广西黄鸡等聚为一类。表明中国地方鸡品种间的同源性较小,BF2基因在不同鸡种上具有丰富的遗传多样性,在第2外显子上C/T突变和第3外显子上A/C突变可作为BF2基因有关抗病的候选SNPs位点。
[Abstract]:The sequence of BF2 (Major histocompatibility complex class I antigen BF2) gene was sequenced by target region capture and sequencing, and the differences in BF2 gene sequence of different chicken breeds were found out. The BF2 gene sequence of Gallus_gallus-5.0 on NCBI website was used as the reference standard. The gene sequences of 25 chicken breeds were analyzed by MEGA6.06 software, SNPs locus and Indel locus were counted, and phylogenetic tree was constructed for analysis. The results showed that there were 22 and 31 sense mutations in exon 2 and 3 of BF2, accounting for 78% of all sense mutations in BF2 gene, indicating that exons 2 and 3 of BF2 gene play an important role and have rich polymorphism. Exon 5, 6, 7 belong to conservative region. 25 chicken breeds can be roughly divided into three categories: Luyuan chicken, Rugao yellow chicken, Liyang chicken and Taihu chicken, etc., Ross chicken and recessive white feather chicken can be grouped into one group, and the other can be divided into three groups: Luyuan chicken, Rugao yellow chicken, Liyang chicken and Taihu chicken. Xueshan chicken and Guangxi yellow chicken are grouped into one class. The results showed that there was little homology among Chinese local chicken breeds, and the BF2 gene had rich genetic diversity in different chicken breeds. C / T mutation in exon 2 and A / C mutation in exon 3 can be used as candidate SNPs sites for disease resistance of BF2 gene.
【作者单位】：扬州大学动物科学与技术学院
【基金】：国家科技支撑计划项目(2015BAD03B03) 江苏高校优势学科建设工程资助项目
【分类号】：S831

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