番茄木虱取食对含Mi-1.2番茄抗氧化酶活性及防御反应相关基因表达的影响

发布时间：2019-03-18 21:25

【摘要】：Mi-1.2基因编码CC-NBS-LRR家族抗病蛋白,对线虫及刺吸式昆虫具有广谱抗性。以番茄木虱和携带Mi-1.2基因的番茄品系‘Motelle’作为研究体系,研究番茄木虱取食对番茄叶片抗氧化酶活性及防御反应相关基因表达的影响。结果表明:番茄木虱的取食显著提高了番茄叶片抗氧化酶POD、CAT、APX等的活性,同时诱导Mi-1.2基因表达;番茄木虱的取食上调SA合成有关基因PAL、SA信号途径标志基因PR-1(P4)及与Mi-1.2功能相关基因WRKY70、WRKY72a、WRKY72b的表达。JA/systemin信号途径和伤害有关的Pin II、TPI-1、PR-6、PR-7、PR-5等及Prosystemin编码基因Psy的表达未发生变化;JA合成途径相关酶编码基因AOS2、Lox D下调表达,AOC的表达没有变化。SA信号途径可能参与Mi-1.2介导的番茄对于刺吸式昆虫番茄木虱的防御反应。另外,Mi-1.2的RNAi沉默不会改变上述基因的m RNA水平,说明Mi-1.2基因产物对这些基因的表达无调控作用。
[Abstract]:Mi-1.2 gene encodes CC-NBS-LRR family resistant proteins and has broad-spectrum resistance to nematodes and spiny insects. Using tomato planthopper and tomato line 'Motelle' with Mi-1.2 gene as research system, the effects of feeding on antioxidant enzyme activity and expression of defense response-related genes in tomato leaves were studied. The results showed that the feeding of tomato planthopper significantly increased the activity of antioxidant enzyme POD,CAT,APX and induced the expression of Mi-1.2 gene in tomato leaves. The feeding of tomato planthopper upregulated the expression of PAL,SA signal pathway marker gene PR-1 (P4) and Mi-1.2 function-related gene WRKY70,WRKY72a,WRKY72b in SA synthesis-related genes. JA / systemin signaling pathway and injury-related Pin II,TPI-1, The expression of PR-6,PR-7,PR-5 and Prosystemin encoding gene Psy did not change. The expression of AOS2,Lox-D was down-regulated by JA synthesis pathway, but the expression of AOC did not change. Sa signaling pathway may be involved in the Mi-1.2-mediated defense response of tomato to sucker-like insect tomato planthopper. In addition, RNAi silencing of Mi-1.2 did not change the m-RNA level of these genes, suggesting that Mi-1.2 gene products had no regulatory effect on the expression of these genes.
【作者单位】：内蒙古大学生命科学学院;
【基金】：国家自然科学基金项目(31460036) 内蒙古大学高层次人才引进科研启动项目(SPH-IMU,30105-125127)
【分类号】：S436.412

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