转入抗旱主效基因水稻苗期抗旱特性鉴定
发布时间：2019-03-19 07:22
【摘要】：发掘和利用抗旱基因资源是水稻抗旱育种的基础。本研究利用PVC管鉴定法,对24份转入抗旱主效基因的材料进行苗期抗旱特性鉴定,设干旱胁迫(DD)和非干旱胁迫(WW)两处理,研究中采用了最大根长、根干物质重、叶干物质重、根叶长比、分蘖数、叶面积、水份消耗量、水份利用率作为鉴定指标。研究结果显示,对于导入抗旱主效基因的材料,经苗期抗旱性鉴定都具有一定的抗旱性,但抗旱的程度有差异。胁迫下,所有材料的最大根长值超过正常生长下的值,处理间差异显著;在根叶长比、分蘖数、水份消耗量、水份利用率上部分材料表现为正增长;在根干物质重、叶干物质重、叶面积上均表现为负增长,处理间差异显著,只是个别材料的值与WW下的生长量差异不显著。鉴定出5份抗旱性表现较好的材料,5份中除IR83376-B-B-71-1外,都转入了两个以上的抗旱基因,说明聚合抗旱基因对提高抗旱性是有效的。鉴定指标间存在较好的相关性并考虑指标值测定的方便准确,最大根长、根叶长比、分蘖数、水份消耗量可作为苗期较好的抗旱性鉴定指标。
[Abstract]:It is the foundation of rice drought resistance breeding to explore and utilize drought resistance gene resources. In this study, the drought resistance characteristics of 24 transgenic materials with major drought resistance genes were identified by PVC method. Two treatments, drought stress (DD) and non drought stress (WW), were used in this study. The maximum root length and root dry matter weight were used in the study. Leaf dry matter weight, root / leaf length ratio, tillering number, leaf area, water consumption and water utilization ratio were used as identification indexes. The results showed that the drought resistance of the materials introduced with major drought resistance genes at seedling stage had certain drought resistance, but the degree of drought resistance was different. Under stress, the maximum root length value of all materials was higher than that under normal growth, and there were significant differences among the treatments, and some of the materials showed positive growth in root / leaf length ratio, tillering number, water consumption and water utilization rate. The root dry matter weight and leaf area showed negative growth, the difference among treatments was significant, but there was no significant difference between the value of individual materials and the growth under WW. Five materials with good drought resistance were identified, and more than two drought resistance genes were transferred into 5 of them except IR83376-B-71-1, indicating that the polymerized drought resistance gene is effective for improving drought resistance. There was a good correlation among the identification indexes and the determination of the index value was convenient and accurate, the maximum root length, root / leaf length ratio, tillering number and water consumption could be used as the identification indexes for drought resistance at seedling stage.
【作者单位】： 澜沧县富邦乡农业服务中心;云南省农业科学院粮食作物研究所;
【基金】：云南省科技计划项目(2015ZA003) 国家863计划项目(2014AA10A603;2011AA10Z104)共同资助
【分类号】：S511
本文编号：2443305
[Abstract]:It is the foundation of rice drought resistance breeding to explore and utilize drought resistance gene resources. In this study, the drought resistance characteristics of 24 transgenic materials with major drought resistance genes were identified by PVC method. Two treatments, drought stress (DD) and non drought stress (WW), were used in this study. The maximum root length and root dry matter weight were used in the study. Leaf dry matter weight, root / leaf length ratio, tillering number, leaf area, water consumption and water utilization ratio were used as identification indexes. The results showed that the drought resistance of the materials introduced with major drought resistance genes at seedling stage had certain drought resistance, but the degree of drought resistance was different. Under stress, the maximum root length value of all materials was higher than that under normal growth, and there were significant differences among the treatments, and some of the materials showed positive growth in root / leaf length ratio, tillering number, water consumption and water utilization rate. The root dry matter weight and leaf area showed negative growth, the difference among treatments was significant, but there was no significant difference between the value of individual materials and the growth under WW. Five materials with good drought resistance were identified, and more than two drought resistance genes were transferred into 5 of them except IR83376-B-71-1, indicating that the polymerized drought resistance gene is effective for improving drought resistance. There was a good correlation among the identification indexes and the determination of the index value was convenient and accurate, the maximum root length, root / leaf length ratio, tillering number and water consumption could be used as the identification indexes for drought resistance at seedling stage.
【作者单位】： 澜沧县富邦乡农业服务中心;云南省农业科学院粮食作物研究所;
【基金】：云南省科技计划项目(2015ZA003) 国家863计划项目(2014AA10A603;2011AA10Z104)共同资助
【分类号】：S511
【相似文献】
相关期刊论文 前1条
1 ;我国科学家成功分离出控制水稻广亲和性状的主效基因S5[J];广东农业科学;2008年09期
，本文编号：2443305
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/2443305.html
最近更新
教材专著
