贵州地方山羊RBP4基因多态性及生物信息学分析

发布时间：2019-03-19 10:20

【摘要】：[目的]对贵州黑山羊和黔北麻羊进行RBP4基因多态位点的筛选,为从分子水平探究RBP4基因对动物繁殖性能的调控机制提供理论依据。[方法]采用DNA混池结合直接测序法分析RBP4基因外显子4在贵州黑山羊和黔北麻羊中的单核苷酸多态性,利用生物信息学软件分析SNP位点对RBP4基因mRNA二级结构的影响。[结果]RBP4基因外显子4在贵州黑山羊和黔北麻羊中均存在4-T214C突变位点,且为同义突变。生物信息学分析结果表明,外显子4-T214C突变导致RBP4基因mRNA二级结构发生变化,自由能降低,结构稳定性增大。[结论]在贵州黑山羊和黔北麻羊中检测到RBP4基因外显子4的1个SNP位点,该突变位点影响了RBP4基因mRNA的二级结构。
[Abstract]:[aim] to screen the polymorphic sites of RBP4 gene in Guizhou Black Sheep and Qianbei Ma Sheep, so as to provide a theoretical basis for exploring the regulatory mechanism of RBP4 gene on animal reproductive performance at molecular level. [methods] single nucleotide polymorphism of exon 4 of RBP4 gene in Guizhou Black Sheep and Northern Guizhou Ma Sheep was analyzed by DNA mixed pool and direct sequencing, and the effect of SNP locus on mRNA secondary structure of RBP4 gene was analyzed by bioinformatics software. [results] there were 4-T214C mutations in exon 4 of RBP4 gene in both Guizhou Black Sheep and Qianbei Ma Sheep, and they were synonymous mutations. The results of bioinformatics analysis showed that the mutation of exon 4-T214C resulted in the change of secondary structure of RBP4 gene mRNA, the decrease of free energy and the increase of structural stability. [conclusion] A SNP site of exon 4 of RBP4 gene was detected in Guizhou Black Sheep and Qianbei Ma Sheep, which affected the secondary structure of mRNA of RBP4 gene.
【作者单位】：遵义市畜牧站;贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室贵州大学动物科学学院贵州省动物遗传育种与繁殖重点实验室;贵州省种畜禽种质测定中心;
【基金】：贵州省科技重大专项子项目(“黔北麻羊高繁殖力配套系建设及产业化示范”,黔科合重大专项字[2013]6008)
【分类号】：S827

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