蔷薇科果树TMT基因家族生物信息学分析
发布时间：2019-03-20 06:56
【摘要】：[目的]本文旨在对蔷薇科果树液泡膜单糖转运蛋白(TMT)基因家族成员进行鉴定,分析其染色体定位、序列特性、进化关系及在白梨中的表达特征,为蔷薇科果树TMT基因功能研究和利用奠定基础。[方法]基于苹果、草莓、梅、桃、白梨、西洋梨和黑树莓7个蔷薇科果树物种的全基因组测序结果,应用生物信息学方法进行序列分析,通过RT-q PCR技术分析白梨TMT基因在各器官组织中和果实不同发育阶段的表达特性。[结果]苹果、草莓、梅、桃、白梨、西洋梨和黑树莓等TMT基因家族成员数量分别为5、2、4、4、6、4、2,各物种TMT基因不均匀地分布在2~4条染色体上。TMT蛋白结构高度保守,均存在11个α螺旋跨膜结构域,氨基酸序列长度700左右,等电点(p I)为4.79~5.93,最大疏水系数为0.312~0.438,为疏水性蛋白,亚细胞定位预测均位于液泡膜。系统进化分析表明,7个蔷薇科果树物种的TMT家族基因明显聚为两组,组Ⅰ中的TMT基因可能为其主要的功能基因。TMT基因家族经历过2次全基因组复制事件,进化过程中主要受纯化选择作用。定量分析发现,白梨中鉴定的Pb TMT1、Pb TMT2、Pb TMT3在成熟叶中和果实膨大期表达量最高,Pb TMT4的表达量随着果实发育不断增高,Pb TMT6的表达量在成熟叶中最高,而在果实发育后期急剧下降。[结论]蔷薇科果树TMT基因家族成员高度保守,主要在液泡膜上起重要功能,不仅在果实糖分积累过程中发挥重要作用,还参与了植物发育和抗逆过程。
[Abstract]:[objective] to identify the family members of vacuolar monosaccharide transporter (TMT) gene in fruit trees of Rosaceae, and to analyze its chromosome localization, sequence characteristics, evolutionary relationship and expression characteristics in white pear. It lays a foundation for the study and utilization of TMT gene in Rosaceae fruit trees. [methods] based on the whole genome sequencing results of 7 species of Rosaceae fruit trees, including apple, strawberry, plum, peach, white pear, western pear and black raspberry, the sequence analysis was carried out by bioinformatics method. RT-q PCR technique was used to analyze the expression characteristics of TMT gene in different organs and tissues and different stages of fruit development. [results] the number of TMT gene family members in apple, strawberry, plum, peach, white pear, western pear and black raspberry was 5, 2, 4, 4, 6, 4, 2, respectively. The TMT gene of each species was unevenly distributed on 2 and 4 chromosomes, and the structure of TMT protein was highly conserved. There are 11 伪-helix transmembrane domains, the amino acid sequence length is about 700, the isoelectric point (p I) is 4.79 ~ 5.93, the maximum hydrophobic coefficient is 0.312 ~ 0.438, and the subcellular localization prediction is located in the vacuolar membrane. Phylogenetic analysis showed that the TMT family genes of 7 species of Rosaceae fruit trees were obviously clustered into two groups, and the TMT gene in group 鈪，
本文编号：2443965
[Abstract]:[objective] to identify the family members of vacuolar monosaccharide transporter (TMT) gene in fruit trees of Rosaceae, and to analyze its chromosome localization, sequence characteristics, evolutionary relationship and expression characteristics in white pear. It lays a foundation for the study and utilization of TMT gene in Rosaceae fruit trees. [methods] based on the whole genome sequencing results of 7 species of Rosaceae fruit trees, including apple, strawberry, plum, peach, white pear, western pear and black raspberry, the sequence analysis was carried out by bioinformatics method. RT-q PCR technique was used to analyze the expression characteristics of TMT gene in different organs and tissues and different stages of fruit development. [results] the number of TMT gene family members in apple, strawberry, plum, peach, white pear, western pear and black raspberry was 5, 2, 4, 4, 6, 4, 2, respectively. The TMT gene of each species was unevenly distributed on 2 and 4 chromosomes, and the structure of TMT protein was highly conserved. There are 11 伪-helix transmembrane domains, the amino acid sequence length is about 700, the isoelectric point (p I) is 4.79 ~ 5.93, the maximum hydrophobic coefficient is 0.312 ~ 0.438, and the subcellular localization prediction is located in the vacuolar membrane. Phylogenetic analysis showed that the TMT family genes of 7 species of Rosaceae fruit trees were obviously clustered into two groups, and the TMT gene in group 鈪，
本文编号：2443965
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