木豆GDSL脂肪酶基因的克隆及表达分析

发布时间：2019-03-20 20:19

【摘要】：【目的】为探明GDSL家族脂肪酶的抗逆机理,【方法】在前期研究基础上,利用RACE技术克隆木豆GDSL脂肪酶基因,并通过荧光定量PCR技术检测其表达特性。【结果】获得1条木豆GDSL脂肪酶基因,命名为Cc GDSL1,该基因含开放阅读框全长1107 bp,编码369个氨基酸;经同源性分析,Cc GDSL1编码的氨基酸序列与豆科植物中GDSL脂肪酶具有较高的相似性。该基因在叶、茎和根中均表达,且叶和茎中表达量显著高于根;干旱胁迫能提高Cc GDSL1的表达量,随干旱的持续表现出先降后升随后趋于稳定的表达模式。【结论】推测Cc GDSL1基因参与木豆应激干旱胁迫。
[Abstract]:[objective] to investigate the anti-stress mechanism of GDSL family lipases, [methods] on the basis of previous studies, the GDSL lipase gene was cloned by RACE. The expression characteristics of GDSL lipase gene were detected by fluorescence quantitative PCR. [results] A GDSL lipase gene was obtained, named Cc GDSL1, which contained an open reading frame (ORF) of 1107 bp, encoding 369 amino acids. The homology analysis showed that the amino acid sequence encoded by Cc GDSL1 was similar to GDSL lipase in legumes. The gene was expressed in leaves, stems and roots, and the expression level in leaves and stems was significantly higher than that in roots. Drought stress could increase the expression level of Cc GDSL1, and showed a stable expression pattern with continuous drought. [conclusion] Cc GDSL1 gene may be involved in drought stress of pigeonpea. [conclusion] it is suggested that Cc GDSL1 gene may play an important role in the drought stress of pigeonpea.
【作者单位】：贵州大学农业生物工程研究院山地植物资源保护与种质创新省部共建教育部重点实验室;贵州大学贵州省森林资源与环境研究中心;
【基金】：国家自然科学基金项目“利用Gene Fishing解析VA菌根增强木豆抗旱能力的分子机理”(30860224) 贵州省科学技术基金项目“在转录水平解析木豆抗旱的分子机理”(20092076)
【分类号】：Q943.2;S567.19

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