青虾Ran基因的克
发布时间：2019-03-21 11:59
【摘要】：本研究应用RACE技术克隆了青虾(Macrobrachium nipponensis)Ran(Ras related nuclear protein,Ras相关核蛋白)基因全长cDNA序列,该基因cDNA全长1191 bp,包括218 bp的5'UTR,648 bp的开放阅读框(ORF),405 bp的3'UTR,编码215个氨基酸。青虾Ran基因属于P-loop-NTPase超级家族,拥有PTZ00132跨结构域,多肽分子量约为24.57 kDa,理论等电点7.13。系统进化树分析表明,在动物界进化中非常保守的青虾Ran多肽与罗氏沼虾(Macrobrachium rosenbergii)聚为一支,具有最近的亲缘关系。使用荧光定量PCR技术检测Ran基因在成体青虾不同组织和卵巢不同发育期的表达差异,结果显示,Ran基因在青虾不同组织中均有表达,其表达量在卵巢中最高,是精巢表达量的7~8倍;随着卵巢的发育,Ran基因的表达水平呈现上升趋势,在卵巢消退期又恢复到较低水平。RNA干扰后,实验组Ran基因表达量显著低于对照组(P0.05),同时卵巢发育关键基因Vg(vitellogenin)在卵巢中的表达量也显著低于对照组(P0.05),推测Ran基因参与雌性卵巢发育过程并对Vg基因的表达起到调控作用。
[Abstract]:In this study, the full-length cDNA sequence of the shrimp (Macrobrachium nipponensis) Ran (Ras related nuclear protein,Ras-associated nucleoprotein (Macrobrachium nipponensis) Ran (Ras related nuclear protein,Ras-associated nucleoprotein) gene was cloned by RACE technique. The full-length cDNA gene consists of 5 UTRs of 218 bp and 3 UTRs of (ORF), 405bp of the open reading frame (ORF) of 648 bp. It encodes 215 amino acids. The Ran gene of shrimps belongs to the P-loop-NTPase superfamily and has a PTZ00132 domain. The molecular weight of the polypeptide is about 24.57 kDa, theoretical isoelectric point 7.13. Phylogenetic tree analysis showed that the very conservative Ran polypeptide of Macrobrachium rosenbergii and Macrobrachium rosenbergii (Macrobrachium rosenbergii) were closely related to each other in the evolution of animal kingdom. The expression of Ran gene was detected by fluorescence quantitative PCR in different tissues of adult shrimps and in different developmental stages of ovary. The results showed that Ran gene was expressed in different tissues of shrimps, and the expression level of Ran gene was the highest in ovary. It was 8 times higher than that of testis. With the development of ovary, the expression level of Ran gene showed an increasing trend, and returned to a lower level in the ovariectomized period. After RNA interference, the expression of Ran gene in the experimental group was significantly lower than that in the control group (P0.05). At the same time, the expression of Vg (vitellogenin), the key gene of ovarian development, was significantly lower than that of the control group (P0.05). It was speculated that Ran gene was involved in the development of female ovary and regulated the expression of Vg gene.
【作者单位】： 南京农业大学无锡渔业学院;中国水产科学研究院淡水渔业研究中心农业部淡水渔业和种质资源利用重点实验室;
【基金】：江苏省重点研发计划(现代农业)重点项目(BE2016308) 中国水产科学研究院基本科研业务费专项项目(2016HY-ZD0402) 国家自然科学基金面上项目(31572617) 江苏省农业科技自主创新基金项目[CX(15)10124] 江苏省水产三新工程(D2015-16) 无锡科学科技发展基金项目(CLE02N1514)
【分类号】：S917.4
，
本文编号：2444911
[Abstract]:In this study, the full-length cDNA sequence of the shrimp (Macrobrachium nipponensis) Ran (Ras related nuclear protein,Ras-associated nucleoprotein (Macrobrachium nipponensis) Ran (Ras related nuclear protein,Ras-associated nucleoprotein) gene was cloned by RACE technique. The full-length cDNA gene consists of 5 UTRs of 218 bp and 3 UTRs of (ORF), 405bp of the open reading frame (ORF) of 648 bp. It encodes 215 amino acids. The Ran gene of shrimps belongs to the P-loop-NTPase superfamily and has a PTZ00132 domain. The molecular weight of the polypeptide is about 24.57 kDa, theoretical isoelectric point 7.13. Phylogenetic tree analysis showed that the very conservative Ran polypeptide of Macrobrachium rosenbergii and Macrobrachium rosenbergii (Macrobrachium rosenbergii) were closely related to each other in the evolution of animal kingdom. The expression of Ran gene was detected by fluorescence quantitative PCR in different tissues of adult shrimps and in different developmental stages of ovary. The results showed that Ran gene was expressed in different tissues of shrimps, and the expression level of Ran gene was the highest in ovary. It was 8 times higher than that of testis. With the development of ovary, the expression level of Ran gene showed an increasing trend, and returned to a lower level in the ovariectomized period. After RNA interference, the expression of Ran gene in the experimental group was significantly lower than that in the control group (P0.05). At the same time, the expression of Vg (vitellogenin), the key gene of ovarian development, was significantly lower than that of the control group (P0.05). It was speculated that Ran gene was involved in the development of female ovary and regulated the expression of Vg gene.
【作者单位】： 南京农业大学无锡渔业学院;中国水产科学研究院淡水渔业研究中心农业部淡水渔业和种质资源利用重点实验室;
【基金】：江苏省重点研发计划(现代农业)重点项目(BE2016308) 中国水产科学研究院基本科研业务费专项项目(2016HY-ZD0402) 国家自然科学基金面上项目(31572617) 江苏省农业科技自主创新基金项目[CX(15)10124] 江苏省水产三新工程(D2015-16) 无锡科学科技发展基金项目(CLE02N1514)
【分类号】：S917.4
，
本文编号：2444911
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/2444911.html
最近更新
教材专著
