抗白粉病基因PmCH1302的遗传分析及染色体定位

发布时间：2019-03-23 18:56

【摘要】：CH1302是以来源于中间偃麦草的八倍体小偃麦TAI7047为桥梁亲本选育的高抗白粉病的小麦新品系,对白粉菌多个流行小种均表现出良好抗性。为了解其抗白粉病基因来源及其在染色体上的位置,对绵阳11×CH1302的F_1、F_2及F_(2∶3)家系进行了遗传分析,推断其抗白粉病基因可能来源于中间偃麦草,暂将其命名为PmCH1302。利用i Select 90K SNP芯片对抗、感病池进行扫描,发现位于2AL染色体上的多态性位点最多,为313个,占全部多态性位点的9.79%,且集中于2AL染色体100~105 c M和150~155 cM两个区域附近。在上述位点选取SSR标记,筛选出3对与Pm CH1302连锁的分子标记,Xwmc522、Xgwm356和Xgwm526,其中Xgwm356和Xgwm526位于Pm CH1302两侧,连锁距离分别为3.1 c M和7.8 cM。利用遗传图谱以及中国春缺体、双端体将PmCH1302定位于小麦2AL染色体上。进一步与位于2AL上的Pm4、Pm50比较发现,PmCH1302可能是位于2AL上的一个新基因或等位基因。
[Abstract]:CH1302 is a new wheat line with high resistance to powdery mildew, which is a new wheat line with high resistance to powdery mildew, TAI7047, which originated from Thinopyrum intermedium, is a bridge parent. It shows good resistance to many popular races of powdery mildew. In order to find out the origin of powdery mildew resistance gene and its position on chromosome, genetic analysis was carried out in the families of Mianyang 11 脳 CH1302, F _ (1), F _ (2) and F _ (2:3). It was concluded that the powdery mildew resistance gene might come from Thinopyrum intermedium. Temporarily name it PmCH1302. By using I Select 90K SNP chip, the susceptible pool was scanned, and it was found that the number of polymorphic sites on 2AL chromosome was the most, accounting for 9.79% of the total polymorphic loci, and it was concentrated near the 100x105 cM and 150 脳 155 cM regions of 2AL chromosome. Three pairs of molecular markers linked to Pm CH1302 were screened out by selecting SSR markers at the above loci. Xgwm356 and Xgwm526 were located on both sides of Pm CH1302 and the linkage distances were 3.1cM and 7.8cM. respectively, in which Xgwm356 and Xgwm526 were located on both sides of Pm CH1302. Using genetic map and Chinese spring deletion, PmCH1302 was mapped to wheat 2AL chromosome. Compared with Pm4,Pm50 located on 2AL, PmCH1302 may be a new gene or allele located on 2AL.
【作者单位】：山西省农业科学院作物科学研究所/作物遗传与分子改良山西省重点实验室;山西大学研究生院;农业部黄土高原作物基因资源与种质创制重点实验室;
【基金】：国家重点研发计划(2016YFD0102004-07) 山西省自然科学基金(201601D102051,201601D011001) 山西省农科院重点项目(YZD1501) 山西省农业攻关项目(20150311001-1) 国家青年基金(31601303)
【分类号】：S435.121.46

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