人参生长素极性运输基因PIN的表达谱研究

发布时间：2019-03-27 13:46

【摘要】：生长素极性运输基因PIN(PIN-FORMED)决定了植物中生长素浓度梯度分布,参与多种发育及次生代谢过程。本研究采用转录组热图聚类方法分析了4年生吉林人参14个不同部位PIN2、PIN3及PIN6的表达谱,结果显示PgPIN2与PgPIN6主要在人参地下根与根茎中表达,PgPIN3在人参的叶子、果实及根中均有很高的表达;采用序列同源比对、RT-PCR扩增测序及生物信息学分析方法,确认了人参3个PIN基因成员PgPIN2、PgPIN3与PgPIN6;采用实时荧光定量PCR技术分析人参不定根、组培苗不同部位的表达模式,发现PgPIN3与PgPIN6在人参不定根根尖表达高,PgPIN2在人参组培苗根部表达高,PgPIN3在人参组培苗的叶中表达高。人参PIN基因的组织特异性表达表明PgPIN2、PgPIN3与PgPIN6对根的生长发育及向性生长很可能具有相应的功能,PIN3对人参植株地上部分的生长具有较重要的意义,可为后期深入挖掘其对人参向性生长等功能奠定基础。
[Abstract]:The polar transport gene PIN (PIN-FORMED) determines the gradient distribution of auxin concentration in plants and participates in a variety of developmental and secondary metabolic processes. In this study, the expression profiles of PIN2,PIN3 and PIN6 in 14 different parts of 4-year-old Ginseng were analyzed by means of transcript group thermal map clustering method. The results showed that PgPIN2 and PgPIN6 were mainly expressed in underground roots and rhizomes of Ginseng, and PgPIN3 was expressed in leaves of Ginseng. High expression was found in fruits and roots. Three PIN gene members of Ginseng, PgPIN2,PgPIN3 and PgPIN6;, were confirmed by sequence homology alignment, RT-PCR amplification and bioinformatics analysis. The expression patterns of adventitious roots and tissue cultured seedlings of Ginseng were analyzed by real-time fluorescence quantitative PCR. It was found that the expression of PgPIN3 and PgPIN6 was high at the root tip of adventitious root of Ginseng, the expression of PgPIN2 was high at the root of adventitious root of Ginseng, and the expression of PgPIN3 was high in the leaves of tissue-cultured seedlings of Ginseng. The tissue-specific expression of PIN gene indicates that PgPIN2,PgPIN3 and PgPIN6 may have corresponding functions to the growth and development of roots, and PIN3 is of great significance to the growth of above-ground parts of Ginseng, and it is very important for the growth of above-ground parts of Ginseng plants. It can lay a foundation for further excavating the function of Ginseng growth in the later stage.
【作者单位】：中国中医科学院中药资源中心;中国农业科学院特产研究所;中国科学院北京基因组研究所;
【基金】：国家自然科学基金资助项目(81603235) 国家杰出青年科学基金项目(81325023) 名贵中药资源可持续利用能力建设项目(2060302) 中国中医科学院中药资源中心自主选题研究项目课题(ZZ2016006)
【分类号】：S567.51

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