RNA干扰A20基因的表达对人乳腺癌细胞MCF-7增殖、凋亡和迁移的影响
发布时间：2019-03-27 15:48
【摘要】：目的利用RNA干扰技术靶向沉默A20基因,对MCF-7细胞株增殖、凋亡和迁移的影响进行研究,探讨A20基因作为乳腺癌治疗靶点的潜在价值。方法人工合成靶向A20基因的siRNA序列和阴性对照(NC)siRNA序列,利用脂质体转染技术将siRNA导入MCF-7细胞株,采用CCK-8细胞增殖实验、Annexin V、7-AAD双染流式细胞术检测凋亡实验和Transwell细胞迁移实验研究沉默A20基因对MCF-7细胞增殖、凋亡和迁移的影响。结果靶向沉默A20基因表达能够有效抑制MCF-7细胞的增殖和迁移,促进细胞凋亡的发生。结论 A20基因在MCF-7细胞的增殖、凋亡和迁移过程中起重要作用,A20基因可能是针对乳腺癌抗肿瘤靶向治疗的潜在靶点。
[Abstract]:Aim to study the effects of RNA interference targeted silencing A20 gene on proliferation, apoptosis and migration of MCF-7 cell line, and to explore the potential value of A20 gene as a therapeutic target for breast cancer. Methods siRNA sequence of targeted A20 gene and (NC) siRNA sequence of negative control were synthesized. SiRNA was transfected into MCF-7 cell line by lipofectamine transfection technique. CCK-8 cell proliferation test, Annexin V, was used. The effects of silencing A20 gene on the proliferation, apoptosis and migration of MCF-7 cells were studied by 7-AAD double staining flow cytometry and Transwell cell migration assay. Results targeted silencing of A20 gene expression could effectively inhibit the proliferation and migration of MCF-7 cells and promote the occurrence of apoptosis. Conclusion A20 gene plays an important role in the proliferation, apoptosis and migration of MCF-7 cells. A20 gene may be a potential target for anti-tumor therapy of breast cancer.
【作者单位】： 安徽医科大学第一附属医院检验科;安徽省亳州市人民医院检验科;
【基金】：安徽省科技创新公共服务平台项目(编号:PT20081011)
【分类号】：R737.9;R730.43
本文编号：2448326
[Abstract]:Aim to study the effects of RNA interference targeted silencing A20 gene on proliferation, apoptosis and migration of MCF-7 cell line, and to explore the potential value of A20 gene as a therapeutic target for breast cancer. Methods siRNA sequence of targeted A20 gene and (NC) siRNA sequence of negative control were synthesized. SiRNA was transfected into MCF-7 cell line by lipofectamine transfection technique. CCK-8 cell proliferation test, Annexin V, was used. The effects of silencing A20 gene on the proliferation, apoptosis and migration of MCF-7 cells were studied by 7-AAD double staining flow cytometry and Transwell cell migration assay. Results targeted silencing of A20 gene expression could effectively inhibit the proliferation and migration of MCF-7 cells and promote the occurrence of apoptosis. Conclusion A20 gene plays an important role in the proliferation, apoptosis and migration of MCF-7 cells. A20 gene may be a potential target for anti-tumor therapy of breast cancer.
【作者单位】： 安徽医科大学第一附属医院检验科;安徽省亳州市人民医院检验科;
【基金】：安徽省科技创新公共服务平台项目(编号:PT20081011)
【分类号】：R737.9;R730.43
【参考文献】
相关期刊论文 前1条
1 解婧;王杰军;;锌指蛋白A20与恶性肿瘤相关性研究的进展[J];临床肿瘤学杂志;2010年01期
【共引文献】
相关期刊论文 前3条
1 史军娜;刘美芹;刘杰;陈玉珍;卢存福;;沙冬青A20/AN1锌指蛋白基因序列及表达分析[J];北京林业大学学报;2012年02期
2 柏力;糜建红;杨明粲;张伟;;锌指蛋白基因抑制人平滑肌细胞表型转化的实验研究[J];局解手术学杂志;2010年04期
3 张新华;南欣荣;;Bcl-2、NF-KB在腮腺腺样囊性癌中的表达及临床意义[J];中国美容医学;2013年12期
相关博士学位论文 前1条
1 杨在启;DNA微阵列方法鉴别七氟醚和丙泊酚麻醉下诱导的基因表达情况[D];山东大学;2014年
相关硕士学位论文 前4条
1 张雷;CD4~+CD25~+调节性T细胞表面α7烟碱型乙酰胆碱受体下游信号通路JAK3-STAT5的研究[D];中国人民解放军军医进修学院;2011年
2 刘庆伟;ZNF139、MRP1、MMP7在人胃癌细胞SGC7901及SGC7901/ADR中的表达及与化疗药物敏感性的关系[D];河北医科大学;2012年
3 王银洁;ZNF139、Survivin与Bcl-2在胃癌及转移淋巴结中的异质性表达[D];河北医科大学;2013年
4 张新华;细胞凋亡相关基因Bcl-2、Bax和核转录因子NF-KB在腮腺腺样囊性癌中的表达及临床意义[D];山西医科大学;2013年
，本文编号：2448326
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/2448326.html
最近更新
教材专著
