基于核基因和叶绿体基因的台湾水韭遗传多样性和遗传结构的分析

发布时间：2019-04-08 15:30

【摘要】：利用核基因和叶绿体基因对台湾水韭(Isoetes taiwanensis Devol)2个居群20个样本的遗传多样性和遗传结构进行了分析,并对台湾水韭的保育提出了建议。核基因的检测结果显示,2个居群共存在18个单倍型,单倍型多样性为0.9842,核苷酸多样性为0.00215,居群间基因流N_m为4.26,居群间分化系数G_(st)值为0.05543;叶绿体DNA的检测结果显示,单倍型数目为6,单倍型多样性为0.6211,核苷酸多样性为0.00326。其中台北居群的单倍型多样性和核苷酸多样性均比金门居群高。核基因和叶绿体基因的AMOVA分析显示,居群内的遗传差异远高于居群间。台湾水韭的核苷酸多样性相对其他水韭属植物较低,可能与台湾水韭的染色体倍型以及狭域分布有关。居群结构的形成可能和基因流以及奠基者效应有关。宜采用原地和迁地保护的方法对居群进行保护。
[Abstract]:The genetic diversity and genetic structure of 20 samples from two populations of (Isoetes taiwanensis Devol) were analyzed by using nuclear gene and chloroplast gene, and some suggestions for conservation of Allium solani were put forward. The results of nuclear gene analysis showed that there were 18 haplotypes in the two populations, the diversity of haplotypes was 0.9842, the diversity of nucleotides was 0.00215, and the flow of gene between populations was 4.26, The G _ (st) value of population differentiation coefficient was 0.05543; The results of chloroplast DNA showed that the number of haplotypes was 6, the diversity of haplotypes was 0.6211, and the diversity of nucleotide was 0.00326. The haplotype diversity and nucleotide diversity of Taipei population were higher than those of Jinmen population. AMOVA analysis of nuclear gene and chloroplast gene showed that the genetic difference among populations was much higher than that among populations. The nucleotide diversity of Allium solanum is lower than that of other species, which may be related to chromosome ploidy and narrow distribution of Allium taiwanensis. The formation of population structure may be related to gene flow and founder effect. The method of in-situ and in-situ protection should be adopted to protect the population.
【作者单位】：武汉大学生命科学学院植物系统学与进化生物学实验室;武汉大学生命科学学院实验教学中心;
【基金】：国家自然科学基金项目(31170203,30870168)~~
【分类号】：Q943.2

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