大豆对大豆花叶病毒SC18株系的抗性遗传和基因定位

发布时间：2019-04-08 12:11

【摘要】：大豆花叶病毒(soybean mosaic virus,SMV)病是我国大豆生产上的一种主要病毒病害,SMV株系SC18是我国东北和南方两大产区的优势株系,在黄淮大豆产区亦零星发生。本研究对5个抗×感杂交组合衍生后代分离群体接种SC18后,发现各组合F_1均抗病,F_2表现3∶1(抗∶感)分离比,F_(2∶3)表现1∶2∶1(抗∶分离∶感)的分离比,表明5个抗病亲本(中作00-683、滨豆95-20、东大2号、中品661和RN-9)对SC18的抗性由一对显性基因控制。抗×抗杂交组合"中作00-683×东大2号"衍生后代分离群体接种SC18,F_2出现15∶1(抗∶感)的分离比,表明中作00-683与东大2号可能各携带一对显性基因,控制对SC18的抗性,且独立遗传;抗×抗杂交组合"中作00-683×滨豆95-20"的F_1、F_2和F_(2∶3)在接种SC18后均未检测出感病株,表明中作00-683与滨豆95-20所携带的对SC18的抗性基因是等位的。利用RN-9×7605重组自交家系将RN-9对SC18的抗病基因Rsc18定位到大豆6号染色体(C2连锁群)SSR标记Satt286和Satt277之间,遗传距离为6.12和4.69 cM。
[Abstract]:Soybean mosaic virus (soybean mosaic virus,SMV) is a major virus disease in soybean production in China. SMV strain SC18 is one of the dominant strains in northeast and south of China, and it also occurs sporadically in Huang-Huai soybean production area. In this study, the isolates derived from five 脳-susceptible hybrids were inoculated with SC18. It was found that all the combinations were resistant to disease, and the segregation ratio was 3:1 (resistance: susceptibility). F2:3 showed a segregation ratio of 1:2:1 (resistance: segregation: susceptibility), indicating that the resistance of five resistant parents (Zhongzuo 0083, Bindou 9520, Dongda 2, Zhongguan661 and RN-9) to SC18 was controlled by a pair of dominant genes. A segregation ratio of 15:1 (resistance: susceptibility) appeared in the segregation population of the derived progenies of "00m683 脳 Dongda 2" in resistant 脳 anti-hybrid combination "Zhongzuo 00m683 脳 Dongda 2", indicating that Zhongzuo 00m683 and Dongda 2 may each carry a pair of dominant genes. The resistance to SC18 was controlled and inherited independently. No susceptible strains were detected in the resistant 脳 anti-hybrid combination ("00x683 脳 Bindou 9520"), and no susceptible strains were detected after inoculation with SC18, and no susceptible strains were detected in the two combinations, F _ (2:3) and F _ (2:3), but no susceptible strains were detected in the two combinations. The results showed that the resistance gene to SC18 carried by Zhongzuo 683 and Bindou 95 was allelic. The resistance gene Rsc18 of RN-9 to SC18 was mapped to the SSR marker Satt286 and Satt277 of soybean chromosome 6 (C2 linkage group) using RN-9 脳 7605 recombinant inbred pedigree. The genetic distance was 6.12 and 4.69 cM..
【作者单位】：南京农业大学大豆研究所/农业部大豆生物学与遗传育种重点实验室/国家大豆改良中心/作物遗传与种质创新国家重点实验室;
【基金】：国家自然科学基金(31671718,31571690) 中央高校基本科研业务费(Y0201600115) 现代农业产业技术体系(CARS-04) 转基因生物新品种培育科技重大专项(2016ZX08004-004) 江苏省现代作物生产协同创新中心(JCIC-MCP)
【分类号】：S435.651

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