探析植物体胚发生的关键基因
发布时间：2019-04-13 13:21
【摘要】：体细胞胚胎是植物离体再生的高效方式及遗传转化的理想受体,也是进行科学研究的理想体系;但是从体细胞转变为胚性细胞的过程是复杂的基因调控网络调控的,在这其中,BABY BOOM/WUSCHEL/LEAFY COTYLEDON/MYB115等转录因子处于体胚调控网络的上游,能够带动下游相关基因的响应从而启动了体细胞胚胎的发生,起着"画龙点睛"的关键作用,是启动体胚发生"按钮"和调控体胚发育的"开关"。本综述分析了上述关键基因在启动体细胞胚胎发生及体胚发育和器官再生等过程中的作用,及探讨了从分子水平调控体胚发生的研究体系。认为,在化学诱导表达体系下操纵BABY BOOM和WUSCHEL基因的表达是启动体胚发生及提高植物再生能力的最有效的调控系统,无论同源和异源表达均表现出明显的功能和诱导体胚发生形成的作用,对于科研和生产均具有重要的价值和意义。
[Abstract]:Somatic embryo is an efficient way of plant regeneration in vitro and an ideal receptor for genetic transformation. It is also an ideal system for scientific research. But the process of transforming from somatic cells to embryonic cells is regulated by complex gene regulatory networks, in which transcription factors such as BABY BOOM/WUSCHEL/LEAFY COTYLEDON/MYB115 are upstream of somatic regulatory networks. It can stimulate the response of the downstream related genes and start the development of somatic embryos, which plays a key role in promoting the button of somatic embryogenesis and the "switch" of regulating the development of somatic embryos, which plays a key role in promoting somatic embryogenesis and regulating the development of somatic embryos, which plays a key role in the development of somatic embryos. This review reviewed the role of these key genes in the initiation of somatic embryogenesis, somatic embryo development and organ regeneration, and discussed the research system for regulating somatic embryogenesis at the molecular level. It is suggested that controlling the expression of BABY BOOM and WUSCHEL genes under the chemical induced expression system is the most effective regulatory system for promoting somatic embryogenesis and enhancing plant regeneration ability. Both homologue and heterologous expression show obvious function and induce the formation of conductor embryo, which is of great value and significance for scientific research and production.
【作者单位】： 四川农业大学园林研究所;四川理工学院生物工程学院;中国农业大学园艺学院;四川农业大学生态林业研究所;
【基金】：四川省教育厅重点项目(15ZA0023) 四川省“突破性竹类花卉育种新材料与方法创新”项目(16ZC2871)共同资助
【分类号】：Q943.2
本文编号：2457602
[Abstract]:Somatic embryo is an efficient way of plant regeneration in vitro and an ideal receptor for genetic transformation. It is also an ideal system for scientific research. But the process of transforming from somatic cells to embryonic cells is regulated by complex gene regulatory networks, in which transcription factors such as BABY BOOM/WUSCHEL/LEAFY COTYLEDON/MYB115 are upstream of somatic regulatory networks. It can stimulate the response of the downstream related genes and start the development of somatic embryos, which plays a key role in promoting the button of somatic embryogenesis and the "switch" of regulating the development of somatic embryos, which plays a key role in promoting somatic embryogenesis and regulating the development of somatic embryos, which plays a key role in the development of somatic embryos. This review reviewed the role of these key genes in the initiation of somatic embryogenesis, somatic embryo development and organ regeneration, and discussed the research system for regulating somatic embryogenesis at the molecular level. It is suggested that controlling the expression of BABY BOOM and WUSCHEL genes under the chemical induced expression system is the most effective regulatory system for promoting somatic embryogenesis and enhancing plant regeneration ability. Both homologue and heterologous expression show obvious function and induce the formation of conductor embryo, which is of great value and significance for scientific research and production.
【作者单位】： 四川农业大学园林研究所;四川理工学院生物工程学院;中国农业大学园艺学院;四川农业大学生态林业研究所;
【基金】：四川省教育厅重点项目(15ZA0023) 四川省“突破性竹类花卉育种新材料与方法创新”项目(16ZC2871)共同资助
【分类号】：Q943.2
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