CBR1基因启动子在猪子宫内膜细胞的功能研究(英文)
发布时间:2019-04-13 18:46
【摘要】:目的:研究CBR1基因启动子在猪子宫内膜细胞的表达调控机制。创新点:发现CBR1基因启动子在猪子宫内膜受到炎性因子核转录因子kappa B(NF-κB)成员p65调控。p65对该启动子具有正向调节作用,但是对于CBR1基因的表达并不是必需的。方法:通过双荧光素酶报告基因载体确定CBR1基因启动子转录活性区,通过染色质免疫沉淀(Ch IP)技术确定p65能够结合CBR1基因启动子,通过超表达和干扰表达实验证实p65对CBR1基因启动子的调控作用。结论:猪CBR1基因启动子-1640/-647区对于其转录活性是必需的,在-1545/-1531区存在p65的结合位点。p65在猪子宫内膜细胞中促进了CBR1基因mRNA的表达,但是干扰p65则不会造成CBR1基因mRNA表达量下降,推断p65不是CBR1基因表达的必需因素。
[Abstract]:Aim: to study the expression and regulation mechanism of CBR1 gene promoter in porcine endometrial cells. Innovation: it is found that the promoter of CBR1 gene is regulated by p65 member of kappa B (NF- kappa B in porcine endometrium. P65 has a positive regulatory effect on the promoter, but it is not necessary for the expression of CBR1 gene. Methods: the transcriptional active region of CBR1 gene promoter was determined by double luciferase reporter gene vector, and p65 could bind to CBR1 gene promoter by chromatin immunoprecipitation (Ch IP) technique. The regulatory effect of p65 on the promoter of CBR1 gene was confirmed by over-expression and interference expression experiments. Conclusion: the transcriptional activity of porcine CBR1 gene promoter-1640 is necessary, and p65 binding site exists in-1545 / 1531 region. P65 promotes the expression of CBR1 gene mRNA in porcine endometrial cells. However, interference with p65 did not result in the decrease of mRNA expression of CBR1 gene, and it was inferred that p65 was not an essential factor in the expression of CBR1 gene.
【作者单位】: Guangdong
【基金】:Project supported by the National Natural Science Foundation of China(No.31201771) the Earmarked Fund for China Agriculture Research System(No.CARS-36)
【分类号】:S828
[Abstract]:Aim: to study the expression and regulation mechanism of CBR1 gene promoter in porcine endometrial cells. Innovation: it is found that the promoter of CBR1 gene is regulated by p65 member of kappa B (NF- kappa B in porcine endometrium. P65 has a positive regulatory effect on the promoter, but it is not necessary for the expression of CBR1 gene. Methods: the transcriptional active region of CBR1 gene promoter was determined by double luciferase reporter gene vector, and p65 could bind to CBR1 gene promoter by chromatin immunoprecipitation (Ch IP) technique. The regulatory effect of p65 on the promoter of CBR1 gene was confirmed by over-expression and interference expression experiments. Conclusion: the transcriptional activity of porcine CBR1 gene promoter-1640 is necessary, and p65 binding site exists in-1545 / 1531 region. P65 promotes the expression of CBR1 gene mRNA in porcine endometrial cells. However, interference with p65 did not result in the decrease of mRNA expression of CBR1 gene, and it was inferred that p65 was not an essential factor in the expression of CBR1 gene.
【作者单位】: Guangdong
【基金】:Project supported by the National Natural Science Foundation of China(No.31201771) the Earmarked Fund for China Agriculture Research System(No.CARS-36)
【分类号】:S828
【相似文献】
相关期刊论文 前10条
1 郝迪,
本文编号:2457832
本文链接:https://www.wllwen.com/kejilunwen/jiyingongcheng/2457832.html
最近更新
教材专著