Ⅰ群禽腺病毒液相基因芯片检测方法的建立
发布时间：2019-04-24 02:07
【摘要】：为了建立一种快速、特异、灵敏的检测I群禽腺病毒的液相基因芯片方法。根据Hexon基因序列设计特异引物,上游引物5'端加TAG序列,下游引物5'端加生物素,进行PCR扩增,将扩增产物与链霉素亲和蛋白、磁珠37℃孵育30 min,通过Luminex200仪器分析。用所建立的液相基因芯片方法进行特异性、灵敏度、重复性及病料样品的检测。结果该方法的特异性强;检测的敏感性可达1×102copies/μL;批内批间的变异系数都在5%以下;检测结果与SYBR Green I荧光PCR方法 100%相符。表明建立的液相基因芯片方法特异性好、灵敏高、重复性好,可用于I群禽腺病毒的检测。
[Abstract]:In order to establish a rapid, specific and sensitive liquid-phase microarray method for detection of group I avian adenoviruses. According to the Hexon gene sequence, specific primers were designed. The upstream primer 5 'end added TAG sequence and downstream primer 5' end added biotin. The amplified product was amplified by PCR. The amplified product was incubated with streptomycin affinity protein at 37 鈩，
本文编号：2464016
[Abstract]:In order to establish a rapid, specific and sensitive liquid-phase microarray method for detection of group I avian adenoviruses. According to the Hexon gene sequence, specific primers were designed. The upstream primer 5 'end added TAG sequence and downstream primer 5' end added biotin. The amplified product was amplified by PCR. The amplified product was incubated with streptomycin affinity protein at 37 鈩，
本文编号：2464016
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