氮肥对甘蔗叶片内生固氮菌nifH基因表达的影响

发布时间：2019-04-24 08:07

【摘要】：为了探究氮肥对甘蔗叶片内生固氮菌nifH基因表达的影响,从RNA和蛋白质水平,采用RT-PCR和Western blot等技术分析施氮和不施氮处理下3个甘蔗品种叶片中nifH基因表达的差异性及对固氮酶相对表达量的影响。结果表明,在大田生长的甘蔗,在施氮肥和对照处理的叶片内,都可检测到固氮菌nifH基因在基因水平和蛋白质水平的表达。施氮处理会抑制或降低叶片内固氮菌的nifH基因的表达,但有利于nifH在蛋白质水平的表达,3个甘蔗品种内生固氮菌nifH基因的相对表达量都是施氮处理下较高,尤其是甘蔗品种ROC22,处理与对照间达到了显著差异。
[Abstract]:In order to investigate the effect of nitrogen fertilizer on the expression of nifH gene in endophytic nitrogen-fixing bacteria of sugarcane leaves, RNA and protein levels were studied. RT-PCR and Western blot techniques were used to analyze the difference of nifH gene expression in leaves of three sugarcane cultivars treated with and without nitrogen application and the effect on the relative expression of nitrogenase. The results showed that the expression of nifH gene was detected both at gene level and protein level in sugarcane grown in field, in the leaves treated with nitrogen fertilizer and control. Nitrogen application could inhibit or decrease the expression of nifH gene of nitrogen-fixing bacteria in leaves, but it was beneficial to the expression of nifH at protein level. The relative expression of nifH gene of endophytic nitrogen-fixing bacteria in three sugarcane varieties was higher than that of nitrogen application. Especially, there was significant difference between sugarcane variety ROC22, treatment and control.
【作者单位】：广西大学农学院亚热带农业生物资源保护与利用国家重点实验室;中国农业科学院甘蔗研究中心农业部广西甘蔗生物技术与遗传改良重点实验室广西农业科学院广西甘蔗遗传改良重点实验室;
【基金】：国家自然科学基金项目(3156100093、31471449) 广西八桂学者和特聘专家专项基金(2013) 国家现代农业产业技术体系广西甘蔗创新团队项目(gjnytxgxcxtd-03) 广西农业科学院团队项目(2015YT02)
【分类号】：S566.1

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