雷公藤鲨烯合酶基因全长cDNA克隆及诱导表达分析
发布时间：2019-04-26 12:46
【摘要】：根据雷公藤悬浮细胞转录组数据设计引物,克隆得到雷公藤鲨烯合酶基因Tw SQS全长c DNA(Gen Bank:KR401220),并对其进行生物信息学分析和诱导表达分析。TwSQS cDNA全长为1 800 bp,开放阅读框为1 242 bp,编码413个氨基酸,编码的蛋白理论等电点为7.94,相对分子质量为47.20 kD。雷公藤悬浮细胞经茉莉酸甲酯(MJ)诱导后,荧光定量PCR表明TwSQS相对表达量明显上升,并于诱导后4 h达到最高。本研究首次克隆得到雷公藤SQS基因,为进一步解析雷公藤三萜类成分生物合成途径奠定基础。
[Abstract]:The full length cDNA (Gen Bank:KR401220 of squalene synthase gene of Tripterygium wilfordii (TwSQS) was cloned according to the data of suspension cell transcript group of Tripterygium wilfordii L. (Tripterygium wilfordii L.). Bioinformatics analysis and induced expression analysis were performed. The full length of Tws cDNA was 1 800 bp, An open reading frame (ORF) of 1 242 bp, encodes 413 amino acids, a theoretical isoelectric point of 7.94 and a relative molecular weight of 47.20 kD.. After the suspension cells were induced by methyl jasmonate (MJ), fluorescence quantitative PCR showed that the relative expression of TwSQS increased significantly, and reached the highest level at 4 h after induction. In this study, the SQS gene of Tripterygium wilfordii was cloned for the first time, which laid a foundation for further analysis of the biosynthesis pathway of triterpenoids.
【作者单位】： 首都医科大学中医药学院;中国中医科学院中药资源中心;
【基金】：国家自然科学优秀青年基金项目(81422053) 国家杰出青年基金项目(81325023)
【分类号】：S567.19;Q943.2
本文编号：2466078
[Abstract]:The full length cDNA (Gen Bank:KR401220 of squalene synthase gene of Tripterygium wilfordii (TwSQS) was cloned according to the data of suspension cell transcript group of Tripterygium wilfordii L. (Tripterygium wilfordii L.). Bioinformatics analysis and induced expression analysis were performed. The full length of Tws cDNA was 1 800 bp, An open reading frame (ORF) of 1 242 bp, encodes 413 amino acids, a theoretical isoelectric point of 7.94 and a relative molecular weight of 47.20 kD.. After the suspension cells were induced by methyl jasmonate (MJ), fluorescence quantitative PCR showed that the relative expression of TwSQS increased significantly, and reached the highest level at 4 h after induction. In this study, the SQS gene of Tripterygium wilfordii was cloned for the first time, which laid a foundation for further analysis of the biosynthesis pathway of triterpenoids.
【作者单位】： 首都医科大学中医药学院;中国中医科学院中药资源中心;
【基金】：国家自然科学优秀青年基金项目(81422053) 国家杰出青年基金项目(81325023)
【分类号】：S567.19;Q943.2
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