普通小麦-十倍体长穗偃麦草衍生新品种抗赤霉病基因的分子鉴别
发布时间：2019-04-27 05:00
【摘要】：【目的】赤霉病(Fusarium head blight,FHB)是世界范围内严重危害小麦生产的病害之一。十倍体长穗偃麦草(Thinopyrum ponticum)具有优良的赤霉病抗性,普通小麦-十倍体长穗偃麦草衍生新品种西农509、西农511和西农529在田间展现出较强的赤霉病抗性。本文旨在对这3个品种的赤霉病抗性基因进行分子鉴别,为它们在小麦赤霉病抗性遗传改良中的应用提供理论依据。【方法】通过赤霉病菌(Fusarium graminearum)人工接种鉴定,明确3个小麦新品种对赤霉病的抗性水平。利用偃麦草E组染色体(臂)第1—7同源群的特异引物对3个普通小麦-十倍体长穗偃麦草衍生新品种及其主要亲本小偃693、小偃597和十倍体长穗偃麦草进行分子鉴定,确定其长穗偃麦草的遗传区段。利用与长穗偃麦草7EL染色体上抗赤霉病基因Fhb7紧密连锁的标记对实验材料进行分析,明确该抗性基因与Fhb7的关系。【结果】鉴定结果表明,西农509、西农529和西农511的赤霉病抗性与中抗对照品种扬麦158的抗性水平相当,表现为中抗。105个长穗偃麦草E基因组特异标记中有7个在3个新品种中均能扩增出长穗偃麦草的特异条带,其中5个标记定位于7EL染色体臂上,2个标记定位于7ES染色体臂上。利用97个定位于7E染色体的特异标记进一步对小偃693、小偃597和3个新品种的遗传片段进行鉴别,结果表明20个标记能在5个长穗偃麦草衍生品种(系)扩增出稳定的十倍体长穗偃麦草的特异条带,其中包括与Fhb7紧密连锁的Xsdau K8、Xsdau K144、Xsdau K27、Xsdau K99、Xcfa2040和Xsdau K116等6个标记(7EL 149.00—7EL 153.77),即表明该区段源自于十倍体长穗偃麦草,跨距约89 c M。然而,已报道的7EL染色体臂末端与Fhb7两侧紧密连锁的分子标记Xsdau K60(7EL 153.77)、Xmag1932(7EL 154.70)、Xcfa2240(7EL 156.27)、Xsdau K66(7EL 158.02)、Xsdau K71(7EL 158.97)和Xsews19(7EL 160.00)等在3个新品种及小偃597中均没检出长穗偃麦草的特异条带。【结论】普通小麦-十倍体长穗偃麦草衍生新品种西农509、西农511和西农529具有较好的赤霉病抗性,携带来自于十倍体长穗偃麦草7E染色体的遗传区段,然而该抗赤霉病基因不同于Fhb7。
[Abstract]:Objective: scab (Fusarium head blight,FHB) is one of the most harmful diseases to wheat production in the world. (Thinopyrum ponticum) showed excellent resistance to scab. Xinong 599, Xinong 511 and Xinong 529 showed strong resistance to scab in the field of Triticum aestivum-Decaploid Elytrigia tenuiflora derived varieties Xinong 509, Xinong 511 and Xinong 529. The aim of this paper is to identify the genes of resistance to scab in these three cultivars, and to provide theoretical basis for their application in genetic improvement of resistance to scab in wheat. [methods] (Fusarium graminearum) inoculation was used to identify the resistance genes of scab in wheat. The resistance level of 3 new wheat varieties to scab was determined. Three new varieties of Triticum aestivum-Decaploid and their main parent parents Xiaoyan 693, Xiaoyan 597 and Decaploid Thinopyrum elongatum were identified by using specific primers of group E chromosome (arm) 1-7 homologue of Thinopyrum triticum, and their main parents were Xiaoyan 693, Xiaoyan 597 and Decaploid Thinopyrum elongatum. The genetic region of Thinopyrum elongatum was determined. A marker closely linked to the resistance gene Fhb7 of Elytrigia elongatum 7EL chromosome was used to identify the relationship between the resistance gene and Fhb7. [results] the identification results showed that Xinong 509, The resistance to scab of Xinong 529 and Xinong 511 was similar to that of medium resistant variety Yangmai 158. Seven of the 105 E genome-specific markers of Thinopyrum elongatum could amplify the specific bands of Thinopyrum elongatum in three new varieties, of which 5 were located on the arm of 7EL chromosome, and 5 of them were located on the arm of chromosome of Elytrigia elongatum. Two markers were located on the 7ES chromosome arm. The genetic fragments of Xiaoyan 693, Xiaoyan 597 and three new varieties were further identified by using 97 specific markers located on chromosome 7e. The results showed that 20 markers could amplify stable decaploid specific bands of Thinopyrum elongatum in 5 varieties (lines), including Xsdau K8, Xsdau K144, Xsdau K27, Xsdau K99, which were closely linked to Fhb7. Six markers (7EL 149.00-7EL 153.77), such as Xcfa2040 and Xsdau K116, indicated that the region originated from Thinopyrum tenuiformis, with a span of about 89 c. However, the reported molecular markers Xsdau K60 (7EL 153.77), Xmag1932 (7EL 154.70), Xcfa2240 (7EL 156.27), Xsdau K66 (7EL 158.02) linked closely to the ends of the 7EL chromosome arm to both sides of the Fhb7. Xsdau K71 (7EL 158.97) and Xsews19 (7EL 160.00) did not detect specific bands of Thinopyrum elongatum in three new varieties and Xiaoyan 597. [conclusion] Xinong 509, a new wheat-decaploid derived variety of Thinopyrum longiflorum, has not been detected. Xinong 511 and Xinong 529 have good resistance to scab and carry a genetic region from chromosome 7e of Thinopyrum tenuiflora. However, this resistance gene is different from that of Fhb7..
【作者单位】： 西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室;陕西省城固县种子管理站;陕西省扶贫开发办公室;
【基金】：国家重点研发计划(2016YFD0102000) 陕西省科技统筹创新工程计划(2015KTZDNY01-01-02)
【分类号】：S435.121.45
本文编号：2466707
[Abstract]:Objective: scab (Fusarium head blight,FHB) is one of the most harmful diseases to wheat production in the world. (Thinopyrum ponticum) showed excellent resistance to scab. Xinong 599, Xinong 511 and Xinong 529 showed strong resistance to scab in the field of Triticum aestivum-Decaploid Elytrigia tenuiflora derived varieties Xinong 509, Xinong 511 and Xinong 529. The aim of this paper is to identify the genes of resistance to scab in these three cultivars, and to provide theoretical basis for their application in genetic improvement of resistance to scab in wheat. [methods] (Fusarium graminearum) inoculation was used to identify the resistance genes of scab in wheat. The resistance level of 3 new wheat varieties to scab was determined. Three new varieties of Triticum aestivum-Decaploid and their main parent parents Xiaoyan 693, Xiaoyan 597 and Decaploid Thinopyrum elongatum were identified by using specific primers of group E chromosome (arm) 1-7 homologue of Thinopyrum triticum, and their main parents were Xiaoyan 693, Xiaoyan 597 and Decaploid Thinopyrum elongatum. The genetic region of Thinopyrum elongatum was determined. A marker closely linked to the resistance gene Fhb7 of Elytrigia elongatum 7EL chromosome was used to identify the relationship between the resistance gene and Fhb7. [results] the identification results showed that Xinong 509, The resistance to scab of Xinong 529 and Xinong 511 was similar to that of medium resistant variety Yangmai 158. Seven of the 105 E genome-specific markers of Thinopyrum elongatum could amplify the specific bands of Thinopyrum elongatum in three new varieties, of which 5 were located on the arm of 7EL chromosome, and 5 of them were located on the arm of chromosome of Elytrigia elongatum. Two markers were located on the 7ES chromosome arm. The genetic fragments of Xiaoyan 693, Xiaoyan 597 and three new varieties were further identified by using 97 specific markers located on chromosome 7e. The results showed that 20 markers could amplify stable decaploid specific bands of Thinopyrum elongatum in 5 varieties (lines), including Xsdau K8, Xsdau K144, Xsdau K27, Xsdau K99, which were closely linked to Fhb7. Six markers (7EL 149.00-7EL 153.77), such as Xcfa2040 and Xsdau K116, indicated that the region originated from Thinopyrum tenuiformis, with a span of about 89 c. However, the reported molecular markers Xsdau K60 (7EL 153.77), Xmag1932 (7EL 154.70), Xcfa2240 (7EL 156.27), Xsdau K66 (7EL 158.02) linked closely to the ends of the 7EL chromosome arm to both sides of the Fhb7. Xsdau K71 (7EL 158.97) and Xsews19 (7EL 160.00) did not detect specific bands of Thinopyrum elongatum in three new varieties and Xiaoyan 597. [conclusion] Xinong 509, a new wheat-decaploid derived variety of Thinopyrum longiflorum, has not been detected. Xinong 511 and Xinong 529 have good resistance to scab and carry a genetic region from chromosome 7e of Thinopyrum tenuiflora. However, this resistance gene is different from that of Fhb7..
【作者单位】： 西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室;陕西省城固县种子管理站;陕西省扶贫开发办公室;
【基金】：国家重点研发计划(2016YFD0102000) 陕西省科技统筹创新工程计划(2015KTZDNY01-01-02)
【分类号】：S435.121.45
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