能量和粗蛋白质水平对肉仔鸡生产性能、养分消化和相关基因表达量的影响

发布时间：2019-04-27 05:32

【摘要】：本试验旨在研究能量和粗蛋白质水平对肉仔鸡生产性能、养分消化利用和相关基因表达量的影响。选用320只健康的21日龄AA肉仔鸡,分为4个处理组,每组8个重复,每个重复10只鸡,分别饲喂由2个粗蛋白水平(16%、18%)和2个能量水平(12.34 MJ/kg、13.17 MJ/kg)组成的4种饲粮。试验期21天。结果表明:(1)能量和粗蛋白水平交互作用显著影响肉仔鸡WBG和F/G(P0.05)。18%CP组肉仔鸡WBG显著高于16%CP组,而其F/G显著高于16%CP组(P0.05);高能饲粮组肉仔鸡WBG显著高于低能组,F/G反之(P0.05)。(2)16%CP组DM、CP和IDE均有高于18%CP组的趋势(P=0.067;P=0.087;P=0.076),且其DM利用率显著高于18%CP组(P0.05),但其N利用率下降了4.93%(P0.05)。18%CP组肉仔鸡的AME和AMEn均显著高于16%CP组(P0.05);与低能组相比,高能组肉仔鸡的AME得到显著提高(P0.05)。(3)随着饲粮能量和粗蛋白质水平的提高,肉仔鸡肠道营养物质转运载体的表达量呈现出不同程度的下降趋势,其中16%CP组肉仔鸡十二指肠bo,+AT和CAT2的表达量显著高于18%CP组,且其肉仔鸡回肠y+LAT2、GLUT5和空肠y+LAT2的表达量显著高于18%CP组(P0.05);低能饲粮组肉仔鸡回肠、空肠PePT1、SGLT1和y+LAT2 mRNA的表达量显著高于高能量饲粮组(P0.05)。由上可见,在本试验条件下,饲粮粗蛋白质水平为18%,能量水平为13.17MJ/kg时,肉仔鸡具有较好的生长性能、饲料转化率;饲粮能量水平为12.34 MJ/kg时,提高了肉仔鸡空肠、回肠SGLT1和回肠y+LAT2以及十二指肠GLUT5 mRNA的表达量;粗蛋白质为18%时,肉仔鸡空肠PePT1和空肠、回肠BoAT mRNA的表达量较高;粗蛋白质为16%时,有助于肉仔鸡空肠和回肠y+LAT2 mRNA的表达。
[Abstract]:The purpose of this study was to study the effects of energy and crude protein levels on performance, nutrient digestion and expression of related genes in broilers. A total of 320 healthy 21-day-old AA broilers were divided into 4 groups with 8 replicas in each group and 10 chickens in each group. The broilers were fed with 2 crude protein levels (16%, 18%) and 2 energy levels (12.34 MJ/kg,), respectively, with two crude protein levels (16%, 18%) and two energy levels (12.34 MJ/kg,). (13.17 MJ/kg) consisting of 4 diets. The trial period was 21 days. The results showed that: (1) the interaction of energy and crude protein significantly affected WBG and Figg in broilers (P0.05). The WBG of broilers in 18%CP group was significantly higher than that in 16%CP group, while the FG level was significantly higher in 18%CP group than that in 16%CP group (P0.05). The WBG of broilers in high-energy diet group was significantly higher than that in low-energy group. On the contrary, DM,CP and IDE in 16%CP group (P0.05). (2) were higher than those in 18%CP group (P < 0. 067) and IDE were higher than those in 18%CP group (P < 0. 067). The utilization ratio of DM was significantly higher than that of 18%CP group (P0.05), but its N utilization rate decreased by 4.93% (P0.05). The AME and AMEn of broilers in 18%CP group were significantly higher than those in 16%CP group (P0.05). Compared with low-energy group, the AME of broilers in high-energy group was significantly increased (P0.05). (- 3). With the increase of dietary energy and crude protein level, the expression of intestinal nutrient transport vector in broilers showed a decreasing trend to different degrees. The expression levels of bo, AT and CAT2 in duodenum of broilers in 16%CP group were significantly higher than those in 18%CP group, and the expression levels of y LAT2,GLUT5 and y LAT2 in ileum of broilers in 18%CP group were significantly higher than those in 18%CP group (P0.05). The expression of PePT1,SGLT1 and y LAT2 mRNA in ileum and jejunum of broilers fed with low energy diet was significantly higher than that of high energy diet group (P0.05). It can be seen from above that the broilers had better growth performance and feed conversion rate when the dietary crude protein level was 18% and the energy level was 13.17MJ/kg. When the dietary energy level was 12.34 MJ/kg, the expression levels of SGLT1, y LAT2 and GLUT5 mRNA in jejunum, ileum, y LAT2 and duodenum of broilers were increased, and the expression of PePT1 and jejunum and BoAT mRNA in ileum were higher when crude protein was 18%. When crude protein was 16%, y LAT2 mRNA expression in jejunum and ileum of broilers was enhanced.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S831.5

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