肺腺癌中PD-1、PD-L1蛋白表达与K-RAS基因突变状态的相关性分析
发布时间：2019-05-06 20:01
【摘要】：目的探讨肺腺癌程序性死亡分子-1(programmed death 1,PD-1)、程序性死亡分子配体-1(programmed death ligand 1,PD-L1)蛋白的表达与K-RAS基因突变的相关性。方法采用免疫组化EnVision两步法检测PD-1、PD-L1蛋白的表达,应用实时荧光定量PCR技术检测K-RAS基因的突变类型。结果肺腺癌组织中PD-1、PD-L1的阳性率均高于良性病变肺组织(P0.01),PD-1、PD-L1蛋白表达与患者性别、年龄、吸烟史、分化程度、淋巴结转移及TNM分期均无相关性(P0.05)。36例肺腺癌样本中发生K-RAS基因突变者8例(22.2%),K-RAS基因突变与患者性别、年龄、吸烟史、分化程度、淋巴结转移及TNM分期均无关(P0.05)。相关分析显示PD-1、PD-L1蛋白表达与K-RAS突变无关(P0.05)。结论 PD-1、PD-L1蛋白在肺腺癌中的表达明显高于良性病变肺组织,但两者表达程度与肺腺癌的临床病理特征及K-RAS基因突变无关。
[Abstract]:Aim to investigate the relationship between the expression of programmed death molecule-1 (programmed death-1), programmed death molecule-1 (programmed death ligand-1), and programmed death molecule-1 (PD-L1) protein and the mutation of K-RAS gene in lung adenocarcinoma. Methods Immunohistochemical EnVision two-step method was used to detect the expression of PD-1,PD-L1 protein, and real-time fluorescence quantitative PCR was used to detect the mutation type of K-RAS gene. Results the positive rate of PD-1,PD-L1 in lung adenocarcinoma was higher than that in benign lung tissues (P0.01). The expression of PD-1,PD-L1 protein was correlated with sex, age, smoking history and differentiation degree. There was no correlation between lymph node metastasis and TNM stage (P0.05). K-RAS gene mutation occurred in 8 cases (22.2%) of 36 lung adenocarcinoma samples, and K-RAS gene mutation was associated with gender, age, smoking history and differentiation degree of the patients, and there was no correlation between K-RAS gene mutation and sex, age, smoking history and differentiation degree of lung adenocarcinoma. There was no significant difference in lymph node metastasis and TNM stage (P0.05). Correlation analysis showed that the expression of PD-1,PD-L1 protein was not associated with K-RAS mutation (P0.05). Conclusion the expression of PD-1,PD-L1 protein in lung adenocarcinoma is significantly higher than that in benign lung tissue, but it is not related to the clinicopathological features of lung adenocarcinoma and the mutation of K-RAS gene.
【作者单位】： 河北省唐山市华北理工大学附属医院病理科;
【基金】：2016年河北省政府资助临床医学优秀人才培养和基础课题研究(361036)
【分类号】：R734.2
[Abstract]:Aim to investigate the relationship between the expression of programmed death molecule-1 (programmed death-1), programmed death molecule-1 (programmed death ligand-1), and programmed death molecule-1 (PD-L1) protein and the mutation of K-RAS gene in lung adenocarcinoma. Methods Immunohistochemical EnVision two-step method was used to detect the expression of PD-1,PD-L1 protein, and real-time fluorescence quantitative PCR was used to detect the mutation type of K-RAS gene. Results the positive rate of PD-1,PD-L1 in lung adenocarcinoma was higher than that in benign lung tissues (P0.01). The expression of PD-1,PD-L1 protein was correlated with sex, age, smoking history and differentiation degree. There was no correlation between lymph node metastasis and TNM stage (P0.05). K-RAS gene mutation occurred in 8 cases (22.2%) of 36 lung adenocarcinoma samples, and K-RAS gene mutation was associated with gender, age, smoking history and differentiation degree of the patients, and there was no correlation between K-RAS gene mutation and sex, age, smoking history and differentiation degree of lung adenocarcinoma. There was no significant difference in lymph node metastasis and TNM stage (P0.05). Correlation analysis showed that the expression of PD-1,PD-L1 protein was not associated with K-RAS mutation (P0.05). Conclusion the expression of PD-1,PD-L1 protein in lung adenocarcinoma is significantly higher than that in benign lung tissue, but it is not related to the clinicopathological features of lung adenocarcinoma and the mutation of K-RAS gene.
【作者单位】： 河北省唐山市华北理工大学附属医院病理科;
【基金】：2016年河北省政府资助临床医学优秀人才培养和基础课题研究(361036)
【分类号】：R734.2
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