发菜RNA-seq转录组分析及耐旱相关基因筛选

发布时间：2019-05-08 07:29

【摘要】：发菜是一种陆生耐旱固氮蓝藻,具有重要的生态和资源价值,但目前发菜的基因组和转录组信息还比较缺乏。本研究采用Illumina Hi Seq TM 2500高通量测序平台对充分吸水和失水后的发菜藻体进行转录组分析,经过拼接组装共获得30 013 560条clean reads,3 100个Unigene,序列平均长度854 nt。将Unigene序列与NR、NT、Swiss-Port、PFAM、GO、COG、KEGG(E-value1.0E~(-5))等数据库进行比对,共有2 874个Unigene获得了注释。通过GO功能分类,917个Unigene映射到GO不同功能节点上;通过Unigene与COG数据库的比对,可将有COG功能的514个Unigene分为20类;通过KEGG pathway分析,有1 220个编码基因参与了135条已知的代谢通路。提示谷胱甘肽、类胡萝卜素、淀粉和蔗糖、ABC转运体等代谢途径的部分基因在失水藻体上调表达,说明这些通路与发菜耐旱性关系密切。因此这些注释信息为今后发菜耐旱基因的挖掘提供了重要依据,同时也为发菜分子生物学的进一步研究提供了试验基础。
[Abstract]:Nostoc is a kind of terrestrial nitrogen-fixing cyanobacteria, which has important ecological and resource value. However, the information of genome and transcript of lettuce is still lacking at present. In this study, the Illumina Hi Seq TM 2500 high throughput sequencing platform was used to analyze the transcripts of fully absorbed and dehydrated seaweed. A total of 30,013,560 clean reads,3 100 Unigene, sequences with an average length of 854 nt. were obtained by splicing and assembling. The Unigene sequence was compared with NR,NT,Swiss-Port,PFAM,GO,COG,KEGG (Evalue1.0E~ (- 5) database, and a total of 2874 Unigene were annotated. Through the classification of GO functions, 917 Unigene are mapped to different functional nodes of GO, and through the comparison of Unigene and COG database, the 514 Unigene with COG function can be divided into 20 categories. By KEGG pathway analysis, 1 220 coding genes were involved in 135 known metabolic pathways. It was suggested that some genes of metabolic pathways such as glutathione carotenoid starch sucrose and ABC transporter were up-regulated in dehydrated algae suggesting that these pathways are closely related to drought tolerance of lettuce. Therefore, these annotated information provide an important basis for the excavation of drought-tolerant genes in the future, and also provide an experimental basis for the further study of molecular biology of the lettuce.
【作者单位】：宁夏大学生命科学学院;
【基金】：国家自然科学基金项目(31360054) 宁夏自然科学基金项目(NZ1608)共同资助
【分类号】：Q943.2

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