抗B亚群禽白血病受体基因变异在三个鸡群体中的分布

发布时间：2019-05-08 06:13

【摘要】：为了研究抗B亚群禽白血病病毒受体基因变异在太行鸡、坝上长尾鸡和商品蛋鸡三个鸡群体中的分布,试验采用PCR技术结合测序技术进行Tvb基因分型,利用POPGene 32程序对鸡群体中Tvb等位基因频率、基因型频率进行统计及哈代温伯格平衡检验。结果表明:在坝上长尾鸡和太行鸡群体中,检测到Tvb基因172CT突变位点,检测的两个等位基因命名为Tvb~(s1)/Tvb~r,太行鸡群体中等位基因频率为0.95/0.05,坝上长尾鸡群体中等位基因频率为0.96/0.04。在坝上长尾鸡和太行鸡鸡群中还检测到184TA/G突变位点,检测的三个等位基因命名为Tvb~(s1)/Tvb~(s3)/Tvb~(s4),太行鸡群体中等位基因频率为0.90/0.02/0.08,坝上长尾鸡群体中等位基因频率为0.95/0.00/0.05。坝上长尾鸡和太行鸡两个群体中的这些突变位点均处于哈代温伯格平衡状态,商品蛋鸡群中未检测到抗性等位基因。说明在三个品种鸡群体中Tvb的抗性等位基因频率很低,具有较高患B亚群、D亚群和E亚群白血病的风险。
[Abstract]:In order to study the distribution of anti-subgroup B avian leukemia virus receptor gene in Taihang chicken, Bashang long-tailed chicken and commercial laying hens, Tvb genotyping was carried out by PCR technique combined with sequencing technique. The allele frequency and genotype frequency of Tvb in chicken population were analyzed by POPGene 32 program and the Hardy Weinberg equilibrium test was carried out. The results showed that the 172CT mutation site of Tvb gene was detected in Bashang long-tailed chicken and Taihang chicken population, and the two alleles were named as Tvb~ (S1) / Tvb~r, Taihang chicken population with a median gene frequency of 0.95 脳 0.05, and the two alleles were named as Tvb~ (S1) / Tvb~r, in Taihang chicken population. The mean gene frequency of Bashang long-tailed chicken population was 0.96 脳 0.04. 184TA/G mutation sites were also detected in the flocks of Bashang long-tailed chicken and Taihang chicken. The three alleles were named Tvb~ (S1) / Tvb~ (S3) / Tvb~ (S4), and the median gene frequency of Taihang chicken population was 0.90? 0.02? The mean gene frequency of Bashang long-tailed chicken population was 0.95% and 0.05%, respectively. These mutation loci were in Hardy Weinberg equilibrium state in two populations of Bashang long-tailed chicken and Taihang chicken, and no resistance alleles were detected in commercial laying hens. The results showed that the frequency of resistance alleles of Tvb was very low in three breeds of chickens, and had a higher risk of leukemia of subgroup B, subgroup D and subgroup E.
【作者单位】：河北农业大学动物科技学院;河北科技师范学院动物科技学院;
【基金】：河北省首批青年拔尖人才支持计划项目(2013-2015) 河北省现代农业产业技术体系蛋鸡产业创新团队项目河北农业大学青年学术带头人支持计划项目
【分类号】：S858.31

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