利用改良SOE-PCR技术定点突变TuMVC4-VPg基因
发布时间：2019-05-14 22:35
【摘要】：芜菁花叶病毒(Turnip mosaic virus,Tu MV)是白菜类蔬菜生产上的重要病害,其中Tu MV VPg基因在Tu MV侵染白菜类蔬菜的过程中起着至关重要的作用,且不同Tu MV株系的致病性不同。本试验采用改良的重叠延伸PCR(SOE-PCR)技术,以Tu MV C4-VPg基因序列为模板,定点突变Tu MV C4-VPg与Tu MV CDN1-VPg基因间5个差异核苷酸位点(决定5个氨基酸差异)。结果表明:通过SOE-PCR技术获得了5个Tu MV C4-VPg基因的单点突变体,即Tu MV C4-VPg-Ⅰ、Tu MV C4-VPg-Ⅱ、Tu MV C4-VPg-Ⅲ、Tu MV C4-VPg-Ⅳ和Tu MV C4-VPg-Ⅴ。
[Abstract]:Turnip mosaic virus (Turnip mosaic virus,Tu MV) is an important disease in cabbage vegetable production, in which Tu MV VPg gene plays an important role in the process of Tu MV infecting cabbage vegetables, and the pathogenicity of different Tu MV strains is different. In this experiment, an improved overlapping extension PCR (SOE-PCR) technique was used to determine the difference of 5 amino acids between site-directed mutant Tu MV C4-VPg and Tu MV CDN1-VPg gene using Tu MV C4-VPg gene sequence as template. The results showed that five single point mutants of Tu MV C4-VPg gene were obtained by SOE-PCR, that is, Tu MV C4 VPgI, Tu MV C4 VPgII, Tu MV C4 VPgIII, Tu MV C4 VPgIV and Tu MV C4 VPgV. The results showed that five single point mutants of VPg鈪，
本文编号：2477099
[Abstract]:Turnip mosaic virus (Turnip mosaic virus,Tu MV) is an important disease in cabbage vegetable production, in which Tu MV VPg gene plays an important role in the process of Tu MV infecting cabbage vegetables, and the pathogenicity of different Tu MV strains is different. In this experiment, an improved overlapping extension PCR (SOE-PCR) technique was used to determine the difference of 5 amino acids between site-directed mutant Tu MV C4-VPg and Tu MV CDN1-VPg gene using Tu MV C4-VPg gene sequence as template. The results showed that five single point mutants of Tu MV C4-VPg gene were obtained by SOE-PCR, that is, Tu MV C4 VPgI, Tu MV C4 VPgII, Tu MV C4 VPgIII, Tu MV C4 VPgIV and Tu MV C4 VPgV. The results showed that five single point mutants of VPg鈪，
本文编号：2477099
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