红掌AaMYB1基因的克

发布时间：2019-05-15 04:21

【摘要】：MYB类转录因子在植物观赏器官色彩形成中发挥关键作用。为了研究红掌中该类转录因子的种类、表达、作用机理等,本研究从红掌中获得了一个MYB转录因子的基因序列,通过反转录多聚酶链式反应(RT-PCR)、生物信息学软件分析、荧光定量PCR检测、构建超表达载体并异源转化烟草等手段,对该转录因子进行了初步研究。结果表明,该转录因子编码基因包含完整编码区,共计912 bp,编码303个氨基酸残基,序列组成与其他物种同源体具有高度相似性;荧光定量分析显示,Aa MYB1在红掌不同组织部位都有表达,但在苞片中表达量最高;获得了12株阳性转化株,形态观察发现转化株营养器官花色素累积程度随基因表达不同而异,但可使所有转化株花器官颜色显著加深。本研究结果为进一步探究MYB转录因子在红掌中调控花色素合成等信息提供了有益参考。
[Abstract]:MYB transcription factors play a key role in color formation of plant ornamental organs. In order to study the species, expression and mechanism of this kind of transcription factors in red palm, a gene sequence of MYB transcription factor was obtained from red palm, which was analyzed by reverse transcriptional polymerase chain reaction (RT-PCR) and bioinformatics software. Fluorescence quantitative PCR (FQ-PCR) was used to detect the transcription factor, and the overexpression vector was constructed and the tobacco was transformed from different sources, and the transcription factor was studied. The results showed that the transcriptional factor encoded gene contained a complete coding region, and a total of 912 bp, encoded 303amino acid residues, and the sequence composition was highly similar to that of other species. Fluorescence quantitative analysis showed that Aa MYB1 was expressed in different tissues of red palm, but the highest expression was found in bracts. Twelve positive transformed strains were obtained. morphological observation showed that the degree of anthocyanin accumulation in vegetative organs of transformed plants varied with gene expression, but the color of floral organs of all transformed plants was significantly deepened. The results of this study provide a useful reference for further exploring the information of MYB transcription factors regulating anthocyanin synthesis in red palm.
【作者单位】：浙江省农业科学院花卉研究开发中心;
【基金】：国家自然科学基金(31200527) 浙江省农业(林木)新品种选育专项(2016C02056-13)
【分类号】：S682.14

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