芥菜型油菜脂肪酸含量的变异、相关性分析及芥酸调控基因FAE1特异引物设计

发布时间：2019-05-16 09:57

【摘要】：芥菜型油菜是我国芸苔属的三大油料作物之一,具有耐旱、抗病虫等优良特性;而我国是芥菜型油菜的重要起源中心,具有丰富的种质资源。该研究从全国各地搜集了34份芥菜型油菜,在贵阳环境条件下种植,其脂肪酸含量(芥酸、油酸、硬脂酸、亚麻酸和亚油酸)表现出丰富的变异,并呈正态分布。结果表明:这些芥菜型油菜种质资源的不同脂肪酸含量间的相关性发现,芥酸和油酸间呈极显著负相关,亚麻酸和硬脂酸呈极显著的正相关,亚麻酸和亚油酸呈现负相关。利用这些材料的脂肪酸含量进行主成分分析,发现绝大部分材料(30份,占88.2%)集中在二维图的特定区域,只有少数其它材料散落在图中其它区域,他们分别是SL63、棱角油菜、T6342和长阳黄芥,这些变异较大的材料在芥菜型油菜的育种中可以发挥特殊作用。此外,运用来自甘蓝型油菜和甘蓝的芥酸调控基因FAE1的已知序列,并设计了FAE1特异引物,而引物在全部34份芥菜型油菜种质资源中均表现出了较好的扩增效果。因此证实芥菜型油菜中至少含有一个FAE1拷贝。该研究结果对于芥菜型油菜育种在我国的开展及其未来的分子育种具有重要的指导意义。
[Abstract]:Mustard rape is one of the three major oil crops in the genus Brass in China, which has the excellent characteristics of drought tolerance and disease and insect resistance, while China is an important origin center of mustard rape and rich in germplasm resources. In this study, 34 rapeseed samples were collected from all over the country and planted under Guiyang environmental conditions. the fatty acid contents (erucic acid, oleic acid, stearic acid, linolenic acid and linoleic acid) showed rich variation and normal distribution. The results showed that there was a very significant negative correlation between erucic acid and oleic acid, a very significant positive correlation between linolenic acid and stearic acid, and a negative correlation between linolenic acid and linoleic acid. Using the fatty acid content of these materials for principal component analysis, it was found that most of the materials (30 samples, 88.2%) were concentrated in the specific area of the two-dimensional map, and only a few other materials were scattered in the other areas of the diagram. They were SL63, respectively. These mutated materials can play a special role in the breeding of mustard rapeseed (Brassica napus L.), T6342 and Changyang yellow mustard. In addition, the known sequences of erucic acid regulatory gene FAE1 from rapeseed and cabbage were used, and FAE1 specific primers were designed, and the primers showed good amplification effect in all 34 rapeseed germline resources. Therefore, it was confirmed that there was at least one copy of FAE1 in mustard rape. The results of this study have important guiding significance for the development of mustard rapeseed breeding in China and its future molecular breeding.
【作者单位】：贵州大学油料作物研究所;杭州市萧山区农业科学技术研究所;杭州市萧山区种子管理站;
【基金】：国家自然科学基金(31560422) 贵州省科学技术基金(黔科合J字[2015]2052号) 国家教育部留学回国人员科研启动基金(教外司留[2015]1098号) 贵州大学博士人才引进基金(贵大人基合字[2014]14号) 贵州省作物学省级重点学科建设计划项目(黔学位合字ZDXK[2014]8号)~~
【分类号】：S565.4

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