慈竹WRKY转录因子基因克隆及其胁迫诱导表达
发布时间：2019-05-16 21:01
【摘要】：为了解慈竹WRKY生物学功能以及通过基因工程手段改良竹类植物。利用已获得慈竹转录组数据,克隆获得2条WRKY转录因子,分别命名为BeWRKY1(Gen Bank:KJ462124)和Be WRKY2(Gen Bank:KJ462125),两者分别编码189和295个氨基酸残基。序列分析表明,两者编码的蛋白均含有一个完整的WRKY superfamily的保守结构域。BeWRKY1属于Ⅱc亚家族;Be WRKY2则与Ta WRKY16和AtWRKY39等聚为一枝,属于Ⅱd亚家族。定量PCR分析表明,Be WRKY1和Be WRKY2分别在茎和完全展开叶中具有较高表达量。200mmol·L~( 1)NaCl和20%PEG 6000胁迫处理激活了Be WRKY1表达,却减少了BeWRKY2的转录积累。结果表明BeWRKY1可能短时间内迅速参与逆境响应;Be WRKY2对干旱和ABA胁迫伤害更为敏感,而可能不参与高盐类胁迫保护反应。
[Abstract]:In order to understand the biological function of Phyllostachys pubescens WRKY and improve bamboo plants by genetic engineering. Based on the obtained data of Zizhu transcriptional group, two WRKY transcription factors, named BeWRKY1 (Gen Bank:KJ462124) and Be WRKY2 (Gen Bank:KJ462125), were cloned, which encoded 189 and 295 amino acid residues, respectively. Sequence analysis showed that the proteins encoded by the two proteins contained a complete conserved domain of WRKY superfamily. BeWRKY1 belonged to the 鈪，
本文编号：2478560
[Abstract]:In order to understand the biological function of Phyllostachys pubescens WRKY and improve bamboo plants by genetic engineering. Based on the obtained data of Zizhu transcriptional group, two WRKY transcription factors, named BeWRKY1 (Gen Bank:KJ462124) and Be WRKY2 (Gen Bank:KJ462125), were cloned, which encoded 189 and 295 amino acid residues, respectively. Sequence analysis showed that the proteins encoded by the two proteins contained a complete conserved domain of WRKY superfamily. BeWRKY1 belonged to the 鈪，
本文编号：2478560
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