青香蕉苹果MdAFS基因序列与表达模式分析

发布时间：2019-05-17 01:36

【摘要】：以青香蕉苹果叶片为材料,研究青香蕉苹果‘White Winter Pearmain’α-法尼烯合酶(α-farnesene synthase,Md AFS)全长基因结构特征及编码蛋白质性质,分析Md AFS基因的诱导表达模式。采用生物信息学软件对Md AFS及其编码蛋白质进行详细分析,应用MEGA 6.0对不同物种来源的AFS构建系统进化树,利用q RT-PCR技术研究Md AFS诱导表达模式。α-法尼烯合酶基因(Gen Bank No:AY563622)全长1 731 bp,由576氨基酸组成,是α螺旋为主要构成元件的混合型蛋白。不同物种的α-法尼烯合酶氨基酸序列相似度低,但α-法尼烯合酶N端RRx8W和C端的H-α1loop具有高度保守性。非生物胁迫和激素信号能诱导Md AFS基因上调表达,且MV、ABA和Me JA诱导该基因上调表达更加明显。α-法尼烯合酶氨基酸序列相似度低,但蛋白三维结构相似;α-法尼烯合酶在蛋白结构和功能上具有单萜合酶特征,初步揭示了非生物胁迫和激素信号能诱导青香蕉苹果α-法尼烯代谢途径的关键酶Md AFS基因上调表达。
[Abstract]:The structural characteristics and coding protein properties of the full-length gene of 'White Winter Pearmain' 伪-farneenase (伪-farnesene synthase,Md AFS) in green banana apple leaves were studied, and the induced expression pattern of Md AFS gene was analyzed. The Md AFS and its encoded proteins were analyzed in detail by bioinformatics software, and the phylogenetic tree of AFS from different species was constructed by MEGA 6.0. The expression pattern induced by Md AFS was studied by Q RT-PCR. The full length 1731 bp, of 伪-farneenase gene (Gen Bank No:AY563622 is composed of 576amino acids and is a mixed protein with 伪 helix as the main component. The amino acid sequence similarity of 伪-farneenase in different species is low, but the N-terminal RRx8W of 伪-farneene enzyme and the H-伪 1loop of C-terminal are highly conserved. Abiotic stress and hormone signal could up-regulate the expression of Md AFS gene, and the up-regulation expression of Md AFS gene induced by MV,ABA and Me JA was more obvious. The amino acid sequence similarity of 伪-farinenase was low, but the three-dimensional structure of protein was similar. 伪-farneene enzyme has the characteristics of monoterpenoids in protein structure and function, which preliminarily reveals that abiotic stress and hormone signal can induce the up-expression of Md AFS gene, the key enzyme of 伪-farinene metabolic pathway in green banana apple.
【作者单位】：山东农业大学生命科学院作物生物学国家重点实验室;
【基金】：国家自然科学基金项目(31370359)
【分类号】：Q943.2;S661.1

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