聚合稻瘟

发布时间：2019-05-18 23:31

【摘要】：结合分子标记辅助轮回选择和田间鉴定的方法,将三黄占2号的抗稻瘟病基因Pi-GD-1(t)和Pi-GD-2(t)(分别简称G1和G2)、CBB23中的抗白叶枯病基因Xa23(简称X)和IR65482-7-216-1-2-B(简称IR65482)的抗褐飞虱基因Bph18(t)(简称B)导入温恢845、温恢117和温恢143等3个中籼恢复系,获得了8个兼抗稻瘟病和褐飞虱聚合系,温恢845-G1-G2-B-4、温恢845-G1-G2-B-5、温恢117-G1-G2-X-B-3、温恢143-G1-G2-B-3、温恢143-G2-X-B-9、温恢143-G2-X-B-10、温恢143-G1-G2-B-11和温恢143-G1-G2-B-37。这些聚合系及其与不育系五丰A的测交种,对稻瘟病和褐飞虱的抗性水平接近或略低于稻瘟病抗性亲本三黄占2号和稻飞虱抗性亲本IR65482。部分改良恢复系如温恢117-G1-G2-X-B-3、温恢143-G2-X-B-9和温恢143-G2-X-B-10及其测交种对白叶枯病表现为抗病或中抗。改良恢复系及其测交种在正常条件下的农艺性状与原始恢复系及其测交种相仿或更优,具有生产应用价值。研究结果表明,Xa23在不同恢复系背景下抗性表达完全,而Pi-GD-1(t)、Pi-GD-2(t)和Bph18(t)对稻瘟病和褐飞虱抗性的改良效果与恢复系的遗传背景有关。
[Abstract]:Combined with molecular marker assisted reincarnation selection and field identification, the rice blast resistance genes Pi-GD-1 (t) and Pi-GD-2 (t) (of Sanhuangzhan 2 were abbreviated as G 1 and G 2, respectively. The brown planthopper resistance genes Xa23 (X) and IR65482-7-216-1-2-B (IR65482) in CBB23 were introduced into three medium indica restorer lines, Wenhui 845, Wenhui 117 and Wenhui 143.The resistant genes Bph18 (t) (B), which are resistant to bacterial blight, were introduced into three medium indica restorer lines, Wenhui 845, Wenhui 117 and Wenhui 143, respectively. Eight fusion lines resistant to rice blast and brown planthopper were obtained, Wenhui 845-G1 鈮，

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