不同引物对和退火温度对盐生植物盐穗木内参基因β-actin扩增效率的影响
发布时间：2019-05-19 10:25
【摘要】：【目的】以盐生植物盐穗木为材料,研究不同引物对和不同退火温度对盐穗木内参基因β-actin扩增效率的影响。【方法】设计扩增β-actin基因的两对引物,标记为β-actin1和β-actin2,分别建立这两对引物扩增盐穗木β-actin基因和特异性引物扩增该物种的过氧化物酶基因POD(盐响应的代表性基因),在不同退火温度下的标准曲线和扩增曲线。【结果】不论55还是58℃退火温度,引物对β-actin1比引物对β-actin2有更好的扩增效率;在55℃退火温度下,引物对β-actin1有更高的扩增效率。在这两个退火温度下,分别以β-actin1和β-actin2引物对扩增β-actin基因作为内参,盐穗木POD基因的相对表达水平具有一定的差异性。【结论】引物和退火温度会影响荧光定量PCR的扩增效率,进而会影响靶基因的相对表达水平。
[Abstract]:[objective] to study the effects of different primer pairs and annealing temperature on the amplification efficiency of 尾-actin gene in halophyte salted wood. [methods] two pairs of primers for amplification of 尾-actin gene were designed. 尾-actin1 and 尾-actin2, were used to amplify 尾-actin gene and specific primer POD (representative gene of salt response), respectively. The standard curve and amplification curve at different annealing temperatures. [results] the amplification efficiency of primer pair 尾-actin1 was better than that of primer pair 尾-actin2 regardless of 55 鈩，
本文编号：2480646
[Abstract]:[objective] to study the effects of different primer pairs and annealing temperature on the amplification efficiency of 尾-actin gene in halophyte salted wood. [methods] two pairs of primers for amplification of 尾-actin gene were designed. 尾-actin1 and 尾-actin2, were used to amplify 尾-actin gene and specific primer POD (representative gene of salt response), respectively. The standard curve and amplification curve at different annealing temperatures. [results] the amplification efficiency of primer pair 尾-actin1 was better than that of primer pair 尾-actin2 regardless of 55 鈩，
本文编号：2480646
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