百脉根ACC氧化酶基因LcACO1的克隆及序列分析

发布时间：2019-05-28 20:54

【摘要】：植物激素乙烯参与植物生长发育多个生理过程的调控。ACC氧化酶是植物乙烯合成途径中的关键合成酶。本研究依据其它物种ACC氧化酶序列信息设计简并引物,并结合RT-PCR和快速扩增c DNA末端技术从牧草百脉根中首次克隆到ACC氧化酶基因的全长c DNA序列,命名为LcACO1。LcACO1全长c DNA为1 012 bp,具有一个924 bp ORF可编码307个氨基酸残基。生物信息学分析表明,LcACO1是含有Fe2+依赖的加氧酶结构域的稳定亲水蛋白,没有信号肽和跨膜结构域,亚细胞定位于细胞质。与其它植物中的ACO蛋白进行同源性比对显示,LcACO1蛋白与鹰嘴豆中ACO蛋白的一致性高达90%。实时荧光定量PCR表明,LcACO1基因在百脉根不同组织器官中差异表达,根中表达量最高,推测其主要在根的生长发育调节方面发挥作用。
[Abstract]:Ethylene, a plant hormone, is involved in the regulation of many physiological processes of plant growth and development. ACC oxidase is the key enzyme in the pathway of plant ethylene synthesis. In this study, degenerate primers were designed according to the sequence information of ACC oxidase in other species, and the full-length c-DNA sequence of ACC oxidase gene was cloned from herbage root for the first time by RT-PCR and rapid amplification of c-DNA terminal technique. The full length c DNA named LcACO1.LcACO1 is 1,012 bp, with a 924 bp ORF encoding 307amino acid residue. Bioinformatics analysis showed that LcACO1 was a stable hydrophilic protein containing Fe2 dependent aerase domain, no signal peptide and transmembrane domain, and subcellular localization in cytoplasm. The homology comparison with ACO protein in other plants showed that the consistency between LcACO1 protein and ACO protein in chickpea was as high as 90%. Real-time fluorescence quantitative PCR showed that LcACO1 gene was differentially expressed in different tissues and organs of Baimai root, and the highest expression was found in roots. It was speculated that LcACO1 gene mainly played a role in the regulation of root growth and development.
【作者单位】：西安文理学院生物与环境工程学院;
【基金】：西安市科技计划项目(CXY1443WL23) 陕西省教育厅科研项目(2015DC031)共同资助
【分类号】：S541.6;Q943.2

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