陆地棉光敏雄性不育基因ys-1的遗传分析与初步定位
发布时间：2019-06-05 07:43
【摘要】：【目的】精细定位和克隆陆地棉光敏雄性不育基因。【方法】从陆地棉中040029的航天诱变后代中选育出新型光敏雄性不育系中9106,以中9106与11个陆地棉材料配制杂交组合,初步分析了中9106的杂种优势。以中9106为母本与陆地棉乐土603构建遗传分离群体F1、F2,分析不育性状的遗传特征。利用集团分离分析法筛选简单序列重复(Simple sequence repeat,SSR),并在包含186个单株的中9106×乐土603 F2群体中用上述SSR初步定位不育基因。【结果】中9106为母本×陆地棉乐土603的F1单株育性均表现正常,而F2群体中的可育单株数∶不育单株数符合3∶1的分离比,推测中9106的不育性状受1对隐性核基因控制。利用集团分离分析法从16 544对SSR中筛选到18对与该不育基因连锁的标记。利用中9106×乐土603的F2群体和18对SSR标记,将不育基因定位于D12染色体,位于标记NAU3442与CGR6339之间,遗传距离均为0.2c M,将该不育基因命名为ys-1。【结论】本研究有助于ys-1的精细定位及其克隆。
[Abstract]:[objective] to localize and clone the photoperiod sensitive male sterile gene of upland cotton. [methods] A new type of Guang Min male sterile line, 9106, was selected from 040029 space mutagenetic progenies of upland cotton, and the hybrid combinations were prepared between Zhong9106 and 11 upland cotton materials. The heterosis of Zhong 9106 was preliminarily analyzed. The genetic segregation populations F1 and F2 were constructed with Zhong 9106 as female parent and Letu 603 of upland cotton, and the genetic characteristics of sterile traits were analyzed. Simple sequence repeat (Simple sequence repeat,SSR was screened by group separation analysis), The sterile genes were initially located by SSR in 9106 脳 Letu 603 F2 population containing 186 plants. [results] the fertility of F1 plants with 9106 female parent 脳 upland cotton 603was normal. However, the number of fertile plants in F2 population: the number of sterile plants per plant was in accordance with the segregation ratio of 3:1, and it was inferred that 9106 of the sterile traits were controlled by a pair of recessive nuclear genes. 18 pairs of markers linked to the sterile gene were screened from 16544 pairs of SSR by group segregation analysis. Using 9106 脳 Letu 603F2 population and 18 pairs of SSR markers, the sterile gene was located on chromosome D12, between marker NAU3442 and CGR6339, and the genetic distance was 0.2cm. Naming the sterile gene ys-1. [conclusion] this study is helpful to the fine mapping and cloning of ys-1.
【作者单位】： 中国农业科学院棉花研究所/棉花生物学国家重点实验室;
【基金】：国家自然科学基金(31501344)
【分类号】：S562
本文编号：2493359
[Abstract]:[objective] to localize and clone the photoperiod sensitive male sterile gene of upland cotton. [methods] A new type of Guang Min male sterile line, 9106, was selected from 040029 space mutagenetic progenies of upland cotton, and the hybrid combinations were prepared between Zhong9106 and 11 upland cotton materials. The heterosis of Zhong 9106 was preliminarily analyzed. The genetic segregation populations F1 and F2 were constructed with Zhong 9106 as female parent and Letu 603 of upland cotton, and the genetic characteristics of sterile traits were analyzed. Simple sequence repeat (Simple sequence repeat,SSR was screened by group separation analysis), The sterile genes were initially located by SSR in 9106 脳 Letu 603 F2 population containing 186 plants. [results] the fertility of F1 plants with 9106 female parent 脳 upland cotton 603was normal. However, the number of fertile plants in F2 population: the number of sterile plants per plant was in accordance with the segregation ratio of 3:1, and it was inferred that 9106 of the sterile traits were controlled by a pair of recessive nuclear genes. 18 pairs of markers linked to the sterile gene were screened from 16544 pairs of SSR by group segregation analysis. Using 9106 脳 Letu 603F2 population and 18 pairs of SSR markers, the sterile gene was located on chromosome D12, between marker NAU3442 and CGR6339, and the genetic distance was 0.2cm. Naming the sterile gene ys-1. [conclusion] this study is helpful to the fine mapping and cloning of ys-1.
【作者单位】： 中国农业科学院棉花研究所/棉花生物学国家重点实验室;
【基金】：国家自然科学基金(31501344)
【分类号】：S562
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