芍药内外花瓣发育形成相关基因片段的生物信息学及组织表达分析

发布时间：2019-06-07 11:19

【摘要】：为揭示芍药内外花瓣形成的分子机制,以托桂型芍药品种‘紫凤羽’为材料,利用转录组测序筛选调控芍药花型发育的关键基因序列片段,首先通过生物信息学方法分析基因片段的蛋白结构与功能以及亚细胞定位,其次利用qPCR检测基因在花瓣中表达情况。结果显示,测序筛选出2个关键基因——AGAMOUS(AG)和MADSbox protein2(MADS2),蛋白分析表明两者均为脂溶亲水性不稳定蛋白,均无剪切信号肽和跨膜结构,为非分泌蛋白,二级结构均以α螺旋(Alpha helix)为主,三维结构模型极为相似,并且包含1个相同的保守功能域—MADS,很少信号肽分泌途径上;qPCR检测显示内瓣AG与MADS2表达量均极显著高于外瓣(P0.01)。芍药AG和MADS2蛋白结构功能、表达水平差异均存在一定的相似性,推测两者可能在芍药花瓣形成过程中发挥相同的生物学功能。
[Abstract]:In order to reveal the molecular mechanism of petal formation inside and outside Paeonia lactiflora, the key gene fragments regulating anther development of Paeonia lactiflora were screened by transcriptional group sequencing. Firstly, the protein structure, function and subcellular localization of the gene fragment were analyzed by bioinformatics method, and then the expression of the gene in petals was detected by qPCR. The results showed that two key genes, AGAMOUS (AG) and MADSbox protein2 (MADS2), were sequenced and screened. Protein analysis showed that both of them were lipophilic hydrophilic unstable proteins with no shear signal peptide and transmembrane structure, and were non-secretory proteins. The secondary structure is mainly 伪-helix (Alpha helix), the three-dimensional structure model is very similar, and contains the same conservative functional domain-MADS, rarely signal peptide secretion pathway. QPCR showed that the expression of AG and MADS2 in the inner flap was significantly higher than that in the outer flap (P 0.01). The structural function and expression level of AG and MADS2 proteins in Paeonia lactiflora were similar. It was speculated that they might play the same biological function in the formation of Paeonia lactiflora petals.
【作者单位】：扬州大学动物科学与技术学院;扬州大学园艺与植物保护学院;
【基金】：国家自然科学基金(31372097;31400592) 江苏省高校自然科学研究重大项目(13KJA210005)
【分类号】：Q943.2;S682.12

【相似文献】