西昌市鸡蛔虫线粒体cyt b和nad1基因的遗传变异分析

发布时间：2019-06-13 19:36

【摘要】：为研究西昌市鸡蛔虫的种内遗传变异和系统发生关系,用线粒体细胞色素b(cyt b)和烟酰胺腺嘌呤二核苷酸脱氢酶亚单位Ⅰ(nad1)基因全序列分析了17个鸡蛔虫西昌分离株的遗传变异,并用cyt b和nad1序列构建鸡蛔虫与其他蛔虫的种系发育关系。测序结果显示:17株鸡蛔虫cyt b和nad1全序列长度分别为1 104 bp和876 bp,碱基变异率分别为02.4%和02.3%;分别检测到10个和7个单倍型,总的单倍型多样性分别为0.897±0.056和0.824±0.064,核苷酸多样性分别为0.004 70±0.001 61和0.004 21±0.001 65,平均遗传距离分别为0.005和0.004。构建的种系发育树均显示17个鸡蛔虫西昌分离株与其他国家/地区的鸡蛔虫分离株聚类形成一个分支,与鸽蛔虫的亲缘关系最近,与其他蛔虫所属的分支相隔较远。研究结果表明鸡蛔虫西昌分离株的遗传变异程度低,且cyt b基因比nad1基因更适合作为研究鸡蛔虫种内遗传变异的分子标记。
[Abstract]:In order to study the intraspecific genetic variation and phylogenetic relationship of Ascaris lumbricoides in Xichang City, the genetic variation of 17 Ascaris lumbricoides isolates was analyzed by using the complete sequence of b (cyt b) and nad1 genes, and the relationship between Ascaris lumbricoides and other Ascaris lumbricoides was constructed by cyt b and nad1 sequences. The sequencing results showed that the full sequence length of cyt b and nad1 of 17 strains of Ascaris lumbricoides were 1.104 bp and 0.2.3%, 10 and 7 haplotypes were detected, the total haplotype diversity was 0.897 卤0.056 and 0.824 卤0.064, the nucleotides diversity was 0.004 70 卤0.001 61 and 0.004 21 卤0.001 65, and the average genetic distance was 0.005 and 0.004, respectively. The constructed species development trees showed that 17 isolates of Ascaris lumbricoides Xichang formed a branch with the isolates of Ascaris lumbricoides in other countries and regions, which were closely related to Ascaris pigeon and far from the branches of other Ascaris lumbricoides. The results showed that the genetic variation of Ascaris lumbricoides Xichang isolate was low, and cyt b gene was more suitable than nad1 gene as a molecular marker to study the intraspecific genetic variation of Ascaris lumbricoides.
【作者单位】：西昌学院动物科学学院;
【基金】：四川省科技厅重点项目(2014NZ0113) 攀西动物疫病检测与防控四川省高校重点实验室基本科研业务费项目(纵341B)
【分类号】：S852.7

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