藤梨根总黄酮对大鼠肝脏组织抗氧化能力及其相关基因表达的影响

发布时间：2019-06-19 00:34

【摘要】：目的对藤梨根总黄酮(TFAR)体内与体外的抗氧化活性及其相关机制进行研究。方法以清除1,1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基能力为指标对TFAR进行体外抗氧化活性实验;以D-半乳糖致衰老大鼠为模型,测定大鼠肝脏组织中丙二醛(MDA)的量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活力及抗氧化相关基因SOD1、SOD2、GPX1 mRNA表达。结果 TFAR对DPPH、ABTS自由基具有良好的清除作用,IC_(50)分别为27.01 mg/mL和29.55 mg/mL。与模型组比较,TFAR各给药组肝脏组织中MDA、SOD、CAT指标均有显著改善(P0.05、0.01),表现在MDA水平降低,SOD、CAT活性升高,并回调到对照组水平以上。TFAR各剂量组对指标的调控作用呈量效关系。与模型组比较,TFAR 3个剂量组均能够上调衰老模型大鼠肝脏组织上清液中SOD1、SOD2、GPX1mRNA的表达,差异显著(P0.05、0.01),且存在一定的剂量依赖关系。结论 TFAR在体外和体内均有抗氧化活性,抗氧化活性与上调大鼠肝脏组织中SOD1、SOD2、GPX1 mRNA表达有关。
[Abstract]:Objective to study the antioxidant activity and related mechanism of total flavonoids (TFAR) in vitro and in vivo. Methods the antioxidant activity of 1, 1-diphenyl-2-trinitrophenylhydrazide (DPPH), 2, 2-biazo-bis (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS) radical was tested in vitro. The content of malondialdehyde (MDA) (MDA), the activity of (SOD), catalase (CAT) and the expression of antioxidant related gene SOD1,SOD2,GPX1 mRNA in liver tissue of aging rats induced by D-galactose were measured. Results TFAR had a good scavenging effect on DPPH,ABTS free radicals. IC_ (50) was 27.01 mg/mL and 29.55 mg/mL., respectively. Compared with the model group, the MDA,SOD,CAT index in the liver tissue of each TFAR group was significantly improved (P0.050.01), which showed that the level of MDA decreased, the activity of SOD,CAT increased, and returned to above the level of the control group. The regulatory effect of each dose group on the index showed a dose-effect relationship. Compared with the model group, all three dose groups of TFAR could up-regulate the expression of SOD1,SOD2,GPX1mRNA in the liver tissue of aging model rats, the difference was significant (P0.05, 0.001), and there was a dose-dependent relationship. Conclusion TFAR has antioxidant activity in vitro and in vivo, which is related to the up-regulation of SOD1,SOD2,GPX1 mRNA expression in rat liver.
【作者单位】：通化师范学院制药与食品科学学院;浙江医药高等专科学校中药学院;
【基金】：国家自然科学基金资助项目(31100254) 吉林省教育厅项目(吉教科合字[2015]第439号)
【分类号】：R285.5

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