Carba NP实验用于筛查肺炎克雷伯菌碳青霉烯酶基因的临床评价
发布时间：2019-06-30 22:44
【摘要】：目的评价Carba NP实验在肺炎克雷伯菌碳青霉烯酶(Klebsiella pneumonia carbapenemases,KPC)基因检测中的临床价值。方法采用Carba NP实验对269株产超广谱β-内酰胺酶肺炎克雷伯菌进行初步筛查,采用PCR扩增其基因,并进行测序。结果 269株肺炎克雷伯菌中对氨苄西林、阿米卡星、头孢吡肟、头孢他啶的耐药率均85%,对哌拉西林/他唑巴坦、头孢唑林耐药率10%;269株肺炎克雷伯菌中,16株对碳青霉烯类抗生素的敏感性降低,Carba NP实验筛查均为阳性,经PCR证实13株细菌均携带KPC基因。结论 Carba NP实验能快速、准确筛查出临床分离鉴定的产KPC型碳青霉烯酶肺炎克雷伯菌,可为医院感染监测和临床合理用药提供实验室依据。
[Abstract]:Objective to evaluate the clinical value of Carba NP assay in detection of carbapenase (Klebsiella pneumonia carbapenemases,KPC gene in Klebsiella pneumoniae. Methods 269strains of Klebsiella pneumoniae producing extended-spectrum 尾-lactamase were screened by Carba NP assay. The genes were amplified by PCR and sequenced. Results the resistance rate of 26 strains of Klebsiella pneumoniae to ampicillin, amikacin, cefepime and ceftazidime was 85%. The resistance rate to piperacillin / tazobactam and cefazolin was 10% 鈮，
本文编号：2508315
[Abstract]:Objective to evaluate the clinical value of Carba NP assay in detection of carbapenase (Klebsiella pneumonia carbapenemases,KPC gene in Klebsiella pneumoniae. Methods 269strains of Klebsiella pneumoniae producing extended-spectrum 尾-lactamase were screened by Carba NP assay. The genes were amplified by PCR and sequenced. Results the resistance rate of 26 strains of Klebsiella pneumoniae to ampicillin, amikacin, cefepime and ceftazidime was 85%. The resistance rate to piperacillin / tazobactam and cefazolin was 10% 鈮，
本文编号：2508315
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