连翘2个DIR基因的分子特征与表达分析

发布时间：2019-07-01 13:57

【摘要】：目的:克隆连翘DIR基因并分析其分子特征和表达特性。方法:克隆两条连翘的DIR基因全长序列,并采用实时荧光定量PCR法对该基因在连翘不同组织以及茉莉酸甲酯诱导下的表达进行分析。结果:克隆得到两个DIR基因(FsDIR1,MF168408;FsDIR2,MF168409),长度分别为558 bp和564 bp,编码氨基酸数目分别为185和187;系统进化树分析表明,FsDIR1属于DIR-a亚家族,FsDIR2属于DIR-b/d亚家族。连翘不同组织实时荧光定量PCR结果表明FsDIR1和FsDIR2在花瓣中表达量最低,而在雌蕊中最高。茉莉酸甲酯诱导后,叶片中连翘苷和连翘酯苷A含量升高,但FsDIR1和FsDIR2的表达量均显著下降。结论:本研究克隆了两个连翘DIR基因,为研究DIR基因在连翘木脂素类化合物生物合成及参与植物的抗性反应中的功能奠定了基础。
[Abstract]:Objective: to clone Forsythia suspensa DIR gene and analyze its molecular characteristics and expression characteristics. Methods: two full-length sequences of Forsythia suspensa DIR gene were cloned and their expression in different tissues of Forsythia suspensa and induced by methyl jasmonate was analyzed by real-time fluorescence quantitative PCR. Results: two DIR genes (FsDIR1,MF168408;FsDIR2,MF168409) were cloned and the amino acids encoded by 558 bp and 564 bp, were 185 and 187, respectively. Phylogenetic tree analysis showed that FsDIR1 belonged to DIR-a subfamily and FsDIR2 belonged to DIR-b/d subfamily. The results of real-time fluorescence quantitative PCR in different tissues of Forsythia suspensa showed that the expression of FsDIR1 and FsDIR2 was the lowest in petals and the highest in pistil. After induction with methyl jasmonate, the contents of phillyrin and phillyrin A in leaves increased, but the expression of FsDIR1 and FsDIR2 decreased significantly. Conclusion: two DIR genes of Forsythia suspensa were cloned in this study, which laid a foundation for studying the function of DIR gene in the biosynthesis of Forsythia suspensa lignans and its involvement in plant resistance.
【作者单位】：河南中医药大学呼吸疾病诊疗与新药研发河南省协同创新中心河南太龙药业股份有限公司
【基金】：中央引导地方科技发展专项资金国家自然科学基金(81603232) 河南中医药大学博士科研基金(BSJJ2015-13)
【分类号】：S567.19

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