转CP4-EPSPs基因大豆杂交后代对草甘膦的抗性水平与遗传背景的相关性
发布时间：2019-07-13 21:22
【摘要】：第一代抗草甘膦转CP4-EPSPs基因大豆GTS40-3-2是国际上应用时间最长、种植面积最大的转基因作物。本文以6份GTS40-3-2衍生的抗草甘膦转基因大豆新品系为亲本,配制4个杂交组合,利用抗性分级法和相对株高法鉴定杂交亲本及其F_(2:3)子代对草甘膦的耐受性差异,分析其抗性水平与遗传背景的相关性。结果表明,以1230 g a.i.hm~( 2)草甘膦喷施处理时,转基因亲本及其F_(2:3)子代的苗期生长受草甘膦抑制不显著,而当喷施浓度提高至3690 g a.i.hm~( 2)和4920 g a.i.hm~( 2)时则抑制作用显著。供试的6个杂交亲本中以ZLHJ06-1568、ZLHJ10-713和ZLHJ06-698对草甘膦的耐受性相对较强,而4个F_(2:3)组合中以ZLHJ10-713×ZLHJ06-698后代在草甘膦喷施后株高受抑制最小,对草甘膦耐受性最强。不同组合后代对草甘膦的耐受性普遍优于其双亲,呈现出杂种优势。各组合后代与亲本之间对草甘膦的耐受性均呈正相关,但由于亲本间互作效应的不同,导致后代抗性水平产生差异。本研究表明草甘膦抗性基因CP4-EPSPs在大豆中的表达水平与其遗传背景相关联,为利用转基因大豆新种质培育转基因大豆新品种过程中目标基因的定向选择提供了参考依据。
[Abstract]:The first generation of glyphosate resistant CP4-EPSPs transgenic soybean GTS40-3-2 is the longest and largest transgenic crop in the world. In this paper, six new glyphosate resistant soybean lines derived from GTS40-3-2 were used as parents to prepare four hybrid combinations. The tolerance of hybrid parents and their F2:3 progenies to glyphosate were identified by resistance classification method and relative plant height method, and the correlation between resistance level and genetic background was analyzed. The results showed that glyphosate did not inhibit the seedling growth of transgenic parents and their F2:3 progenies when sprayed with 1230 g a.i.hm2 glyphosate, but the inhibitory effect was significant when the spraying concentration increased to 3690 g a.i.hm2 and 4920 g a.i.hm2.The effect of glyphosate on the seedling growth of transgenic parents and their F2:3 progenies was not significant when the spraying concentration was increased to 3690 g a.i.hm2 and 4920 g a.i.hm2.The effect of glyphosate on the seedling growth of transgenic parents and their F2:3 progenies was not significant. Among the six hybrid parents tested, ZLHJ06-1568,ZLHJ10-713 and ZLHJ06-698 had relatively strong tolerance to glyphosate, while among the four F2:3 combinations, ZLHJ10-713 脳 ZLHJ06-698 progenies had the least inhibition on plant height and the strongest tolerance to glyphosate after glyphosate spraying. The tolerance of different combinations to glyphosate was generally better than that of their parents, showing heterosis. There was a positive correlation between the tolerance of progenies and parents to glyphosate, but the resistance level of progenies was different due to the different interaction effect between parents. The results showed that the expression level of glyphosate resistance gene CP4-EPSPs in soybean was related to its genetic background, which provided a reference for the targeted selection of target genes in the process of breeding new transgenic soybean varieties.
【作者单位】： 呼伦贝尔市农业科学研究所/国家大豆改良中心呼伦贝尔分中心;中国农业科学院作物科学研究所/国家农作物基因资源与遗传改良重大科学工程/农业部北京大豆生物学重点实验室;东北农业大学;黑龙江省农业科学院大豆研究所;
【基金】：国家转基因生物新品种培育重大专项(ZX2008004-001,ZX2011004-001,ZX2013004-001,ZX2014004-001)资助 supported by the Major Project of China on New Varieties of GMO Cultivation(ZX2008004-001,ZX2011004-001,ZX2013004-001,ZX2014004-001)
【分类号】：S565.1
本文编号：2514294
[Abstract]:The first generation of glyphosate resistant CP4-EPSPs transgenic soybean GTS40-3-2 is the longest and largest transgenic crop in the world. In this paper, six new glyphosate resistant soybean lines derived from GTS40-3-2 were used as parents to prepare four hybrid combinations. The tolerance of hybrid parents and their F2:3 progenies to glyphosate were identified by resistance classification method and relative plant height method, and the correlation between resistance level and genetic background was analyzed. The results showed that glyphosate did not inhibit the seedling growth of transgenic parents and their F2:3 progenies when sprayed with 1230 g a.i.hm2 glyphosate, but the inhibitory effect was significant when the spraying concentration increased to 3690 g a.i.hm2 and 4920 g a.i.hm2.The effect of glyphosate on the seedling growth of transgenic parents and their F2:3 progenies was not significant when the spraying concentration was increased to 3690 g a.i.hm2 and 4920 g a.i.hm2.The effect of glyphosate on the seedling growth of transgenic parents and their F2:3 progenies was not significant. Among the six hybrid parents tested, ZLHJ06-1568,ZLHJ10-713 and ZLHJ06-698 had relatively strong tolerance to glyphosate, while among the four F2:3 combinations, ZLHJ10-713 脳 ZLHJ06-698 progenies had the least inhibition on plant height and the strongest tolerance to glyphosate after glyphosate spraying. The tolerance of different combinations to glyphosate was generally better than that of their parents, showing heterosis. There was a positive correlation between the tolerance of progenies and parents to glyphosate, but the resistance level of progenies was different due to the different interaction effect between parents. The results showed that the expression level of glyphosate resistance gene CP4-EPSPs in soybean was related to its genetic background, which provided a reference for the targeted selection of target genes in the process of breeding new transgenic soybean varieties.
【作者单位】： 呼伦贝尔市农业科学研究所/国家大豆改良中心呼伦贝尔分中心;中国农业科学院作物科学研究所/国家农作物基因资源与遗传改良重大科学工程/农业部北京大豆生物学重点实验室;东北农业大学;黑龙江省农业科学院大豆研究所;
【基金】：国家转基因生物新品种培育重大专项(ZX2008004-001,ZX2011004-001,ZX2013004-001,ZX2014004-001)资助 supported by the Major Project of China on New Varieties of GMO Cultivation(ZX2008004-001,ZX2011004-001,ZX2013004-001,ZX2014004-001)
【分类号】：S565.1
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1 刘吉焘;马晓杰;狄佳春;陈旭升;;棉花草甘膦抗性基因CP4-EPSPS的初步定位[J];江苏农业学报;2013年03期
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