大肠杆菌TorR蛋白对基因entA、sufA、speA和yhiD的表达调控作用
发布时间：2019-08-09 11:37
【摘要】：细菌二组分体能感受外界环境因子的变化,并调节一些基因的表达来使细菌适应环境变化。我们之前的研究发现大肠杆菌二组分体TorR/Tor S中的应答蛋白TorR聚集成焦点(TorR focus)定位在细胞旧极,并且TorR焦点能够以依赖于细胞周期的方式与染色体DNA共定位,而且通过这种方式来调节一些基因的表达。这些基因包含Fe~(2+)转运相关基因,铁硫簇组装相关基因等。本研究旨在探究TorR蛋白调控基因表达的机制。发现TorR在细胞周期特定时期抑制entA、sufA、speA和yhiD基因的表达,并且这种抑制作用是可能通过结合到entA、sufA、speA和yhiD的启动子区域来实现的。另外,sufA基因的表达可能同时受到TorR和Fur蛋白的调控,但是体内试验证明TorR与Fur蛋白之间并不能发生相互作用。
[Abstract]:The two components of bacteria can feel the changes of external environmental factors and regulate the expression of some genes to make bacteria adapt to environmental changes. Our previous studies have found that the response protein TorR in E. coli TorR/Tor S aggregates into a focal (TorR focus) located in the old pole of the cell, and the TorR focus can co-locate with chromosome DNA in a cell cycle dependent manner, and in this way regulate the expression of some genes. These genes include Fe~ (2) transport related genes, iron sulfur cluster assembly related genes and so on. The purpose of this study was to explore the mechanism of TorR protein regulating gene expression. It was found that TorR inhibited the expression of entA,sufA,speA and yhiD genes during a specific period of cell cycle, and this inhibition may be achieved by binding to the promoter regions of entA,sufA,speA and yhiD. In addition, the expression of sufA gene may be regulated by both TorR and Fur proteins, but in vivo experiments have shown that there is no interaction between TorR and Fur proteins.
【作者单位】： 内蒙古自治区人民医院
【基金】：内蒙古自治区自然科学基金项目(2014MS0803) 内蒙古自治区人民医院院内基金项目(2016105)共同资助
【分类号】：Q78
本文编号：2524764
[Abstract]:The two components of bacteria can feel the changes of external environmental factors and regulate the expression of some genes to make bacteria adapt to environmental changes. Our previous studies have found that the response protein TorR in E. coli TorR/Tor S aggregates into a focal (TorR focus) located in the old pole of the cell, and the TorR focus can co-locate with chromosome DNA in a cell cycle dependent manner, and in this way regulate the expression of some genes. These genes include Fe~ (2) transport related genes, iron sulfur cluster assembly related genes and so on. The purpose of this study was to explore the mechanism of TorR protein regulating gene expression. It was found that TorR inhibited the expression of entA,sufA,speA and yhiD genes during a specific period of cell cycle, and this inhibition may be achieved by binding to the promoter regions of entA,sufA,speA and yhiD. In addition, the expression of sufA gene may be regulated by both TorR and Fur proteins, but in vivo experiments have shown that there is no interaction between TorR and Fur proteins.
【作者单位】： 内蒙古自治区人民医院
【基金】：内蒙古自治区自然科学基金项目(2014MS0803) 内蒙古自治区人民医院院内基金项目(2016105)共同资助
【分类号】：Q78
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1 姚远;乔佳鑫;李静;李慧;莫日根;;大肠杆菌TorS/TorR二组分体应答蛋白TorR对DNA复制起始的影响[J];遗传;2015年03期
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1 谭国强;丁焕根;吕建新;;IscA和SufA蛋白在大肠杆菌铁硫簇合成中的功能研究[A];“细胞活动 生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会论文摘要集[C];2011年
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