仿刺参纤维胶凝蛋白基因的分子特征及表达分析
发布时间：2019-08-10 07:39
【摘要】：纤维胶凝蛋白是非特异性免疫系统中的重要分子。通过转录组测序及cDNA末端快速克隆技术得到一条仿刺参纤维胶凝蛋白基因的全长cDNA序列,并将其编码的蛋白命名为仿刺参纤维胶凝蛋白-1。获得的基因cDNA全长为1951bp,其中5′-末端非翻译区为397bp,3′-末端非翻译区为666bp,开放阅读框为888bp,编码295个氨基酸,N端16个氨基酸为信号肽,信号肽后面有两个G-X-Y重复序列,C端为纤维蛋白素原结构域。采用实时荧光定量PCR方法分析了仿刺参纤维胶凝蛋白-1基因在仿刺参幼参不同组织及细菌脂多糖刺激后的时序表达规律,结果显示仿刺参纤维胶凝蛋白-1基因在仿刺参的肠道、呼吸树、体腔细胞和体壁均有表达,且肠道的表达量最高;脂多糖刺激后,4种组织的仿刺参纤维胶凝蛋白-1基因表达量均有变化,且以肠道和体壁表达量的变化最为显著;此外,仿刺参纤维胶凝蛋白-1基因在仿刺参4种组织中的表达量变化具有不同的时序性,表明仿刺参纤维胶凝蛋白-1可能在仿刺参免疫应答中起重要作用。
[Abstract]:Fibrin is an important molecule in the nonspecific immune system. A full-length cDNA sequence of fiber cementitious protein gene of Panax quinquefolium was obtained by sequencing of transcriptional group and rapid cloning of cDNA terminal, and the protein encoded by it was named as fibro-cementitious protein of Panax quinquefolium. The full length of the gene cDNA was 1951bp. the untranslated region was 397bp, the untranslated region was 666bp, the open reading frame was 888bp. the N-terminal 16 amino acids were signal peptides, and there were two G-X-Y repeats behind the signal peptides, and the C-terminal was fibrinogen domain. The untranslated region was 397bp, the open reading frame was 888bp, the N-terminal 16 amino acids were signal peptides, and the C-terminal untranslated region was 397 bp. the C-terminal untranslated region was 666bp. The temporal expression of fibronectin-1 gene in different tissues and bacteria of Panax quinquefolium stimulated by lipopolysaccharide was analyzed by real-time fluorescence quantitative PCR. The results showed that fibronectin-1 gene was expressed in the intestine, respiratory tree, somatic cavity cells and body wall of Panax quinquefolium, and the expression of fibronectin-1 gene in intestinal tract was the highest. After lipopolysaccharide stimulation, the expression of fibronectin-1 gene in the four tissues of Panax quinquefolium changed, especially in intestine and body wall. In addition, the expression of fibronectin-1 gene in four tissues of Panax quinquefolium had different timing, indicating that fibronectin-1 may play an important role in the immune response of Panax quinquefolium.
【作者单位】： 辽宁省海洋水产科学研究院辽宁省海洋水产分子生物学重点实验室;
【基金】：辽宁省科技计划项目(2014203006) 辽宁省海洋与渔业厅项目(201605) 大连市科技计划项目(2013J21DW025)
【分类号】：S917.4
，
本文编号：2525073
[Abstract]:Fibrin is an important molecule in the nonspecific immune system. A full-length cDNA sequence of fiber cementitious protein gene of Panax quinquefolium was obtained by sequencing of transcriptional group and rapid cloning of cDNA terminal, and the protein encoded by it was named as fibro-cementitious protein of Panax quinquefolium. The full length of the gene cDNA was 1951bp. the untranslated region was 397bp, the untranslated region was 666bp, the open reading frame was 888bp. the N-terminal 16 amino acids were signal peptides, and there were two G-X-Y repeats behind the signal peptides, and the C-terminal was fibrinogen domain. The untranslated region was 397bp, the open reading frame was 888bp, the N-terminal 16 amino acids were signal peptides, and the C-terminal untranslated region was 397 bp. the C-terminal untranslated region was 666bp. The temporal expression of fibronectin-1 gene in different tissues and bacteria of Panax quinquefolium stimulated by lipopolysaccharide was analyzed by real-time fluorescence quantitative PCR. The results showed that fibronectin-1 gene was expressed in the intestine, respiratory tree, somatic cavity cells and body wall of Panax quinquefolium, and the expression of fibronectin-1 gene in intestinal tract was the highest. After lipopolysaccharide stimulation, the expression of fibronectin-1 gene in the four tissues of Panax quinquefolium changed, especially in intestine and body wall. In addition, the expression of fibronectin-1 gene in four tissues of Panax quinquefolium had different timing, indicating that fibronectin-1 may play an important role in the immune response of Panax quinquefolium.
【作者单位】： 辽宁省海洋水产科学研究院辽宁省海洋水产分子生物学重点实验室;
【基金】：辽宁省科技计划项目(2014203006) 辽宁省海洋与渔业厅项目(201605) 大连市科技计划项目(2013J21DW025)
【分类号】：S917.4
，
本文编号：2525073
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