OsmC基因在豌豆根瘤菌抗氧化和共生中的功能
发布时间：2019-08-11 16:34
【摘要】：为研究OsmC基因在豌豆根瘤菌抗氧化和共生固氮中的功能,以豌豆根瘤菌RL3841的OsmC基因为研究对象,通过同源重组获得了OsmC基因突变株RLOsmC.结果表明:OsmC基因突变不影响豌豆根瘤菌的自生生长能力,但对无机氧化物H_2O_2和低浓度有机氧化物氢过氧化枯烯(CuOOH)都十分敏感.突变株RLOsmC感染豌豆宿主能形成红色有效根瘤,但是其固氮酶活降低了18.3%.OsmC基因的表达不受H_2O_2的诱导,但在7 d根瘤类菌体中表达量显著上调.表明根瘤菌OsmC基因在共生和抗氧化功能中发挥了重要的作用.
[Abstract]:In order to study the function of OsmC gene in antioxidant and symbiotic nitrogen fixation of Rhizobium pea, the mutant RLOsmC. of OsmC gene was obtained by homologous recombination with the OsmC gene of Rhizobium pea RL3841 as the object of study. The results showed that the mutation of OsmC gene did not affect the autogenic growth ability of Rhizobium pea, but it was very sensitive to inorganic oxide H_2O_2 and low concentration organic oxide (CuOOH). Red effective nodules were formed in pea hosts infected with mutant RLOsmC, but the nitrogenase activity decreased the expression of 18.3%.OsmC gene not induced by H_2O_2, but was significantly up-regulated in nodular bacteria on the 7th day. It is suggested that the OsmC gene of Rhizobium plays an important role in symbiosis and antioxidant function.
【作者单位】： 中南民族大学生命科学学院;
【基金】：湖北省自然科学基金资助项目(2014CFB914) 中央高校基本科研业务费专项(CZW15112)
【分类号】：Q939.114
本文编号：2525402
[Abstract]:In order to study the function of OsmC gene in antioxidant and symbiotic nitrogen fixation of Rhizobium pea, the mutant RLOsmC. of OsmC gene was obtained by homologous recombination with the OsmC gene of Rhizobium pea RL3841 as the object of study. The results showed that the mutation of OsmC gene did not affect the autogenic growth ability of Rhizobium pea, but it was very sensitive to inorganic oxide H_2O_2 and low concentration organic oxide (CuOOH). Red effective nodules were formed in pea hosts infected with mutant RLOsmC, but the nitrogenase activity decreased the expression of 18.3%.OsmC gene not induced by H_2O_2, but was significantly up-regulated in nodular bacteria on the 7th day. It is suggested that the OsmC gene of Rhizobium plays an important role in symbiosis and antioxidant function.
【作者单位】： 中南民族大学生命科学学院;
【基金】：湖北省自然科学基金资助项目(2014CFB914) 中央高校基本科研业务费专项(CZW15112)
【分类号】：Q939.114
【相似文献】
相关期刊论文 前9条
1 杨永友;;2,4-D引导豌豆根瘤菌进入棉花形成根瘤的研究[J];植物医生;2011年03期
2 黄世贞;M.A.Djordjevic;B.G.Rolfe;;豌豆根瘤菌转移接合子侵染的白三叶草根部形态特征研究[J];Journal of Integrative Plant Biology;1992年02期
3 ;固氮作用[J];生物技术通报;1987年07期
4 王惠贤,尤崇杓,R.C.Van den Bos;由质粒介导的豌豆根瘤菌(Rhizobium Leguminosarum)Nif D::Tn5向棕色固氮菌(Azotobacter vinelandii)的转移[J];核农学报;1988年01期
5 ;固氮作用[J];生物技术通报;1988年06期
6 ;生物固氮[J];生物技术通报;1994年02期
7 韦革宏,贺学礼,郭杰,朱铭莪,陈文新;豌豆根瘤菌与新疆中华根瘤菌原生质体的属间融合研究[J];生物工程学报;2001年05期
8 ;固氮作用[J];生物技术通报;1989年06期
9 ;[J];;年期
相关硕士学位论文 前1条
1 周艳琳;豌豆根瘤菌谷氧还蛋白基因grxs的功能研究[D];中南民族大学;2015年
，本文编号：2525402
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/2525402.html
最近更新
教材专著
