桥本甲状腺炎BRAFV600E基因突变与甲状腺乳头状癌相关性研究

发布时间：2019-08-12 17:19

【摘要】：目的探讨桥本甲状腺炎BRAFV600E基因突变与甲状腺乳头状癌发生之间的关系。方法回顾性分析2011年1月至2014年12月期间,在我院行甲状腺切除手术的桥本甲状腺炎合并甲状腺癌患者42例与单纯桥本甲状腺炎患者60例,对两组患者的全部病理标本基因组DNA进行纯化并测序。所得结果与标准核苷酸序列进行比对分析并统计BRAFV600E基因突变情况。结果所有42例纳入研究的桥本甲状腺炎并甲状腺乳头状癌患者中,发生BRAFV600E突变的病例数与无BRAFV600E突变的病例数分别为31例和11例,分别占73.8%、26.1%。所有对照组60例纳入研究的单纯桥本甲状腺炎患者中,发生BRAFV600E突变的病例数与无BRAFV600E突变的病例数分别为2例和58例,分别占3.3%、96.6%,两者差异有统计学差异有统计学意义(P0.05)。结论 BRAFV600E基因突变在桥本甲状腺炎并甲状腺癌患者较单纯桥本甲状腺炎患者更加常见,BRAF600E突变可能是桥本甲状腺炎合并甲状腺癌的高危因素。从而为早期诊断甲状腺癌提供帮助。
[Abstract]:Objective to investigate the relationship between BRAFV600E gene mutation and the occurrence of thyroid papilla carcinoma in Hashimoto's thyroiditis. Methods from January 2011 to December 2014, 42 patients with Hashimoto's thyroiditis complicated with thyroid cancer and 60 patients with simple Hashimoto's thyroiditis underwent thyroidectomy in our hospital. The genomic DNA of all pathological specimens of the two groups was purified and sequenced. The results were compared with the standard nucleotides and the mutation of BRAFV600E gene was counted. Results among all the 42 patients with Hashimoto's thyroiditis complicated with papilla thyroid carcinoma, the number of cases with BRAFV600E mutation and the number of cases without BRAFV600E mutation were 31 and 11, respectively, accounting for 73.8% and 26.1%, respectively. In all the control group, there were 2 cases of BRAFV600E mutation and 58 cases without BRAFV600E mutation, accounting for 3.3% and 96.6%, respectively. there was significant difference between the two groups (P 0.05). Conclusion BRAFV600E gene mutation is more common in patients with Hashimoto thyroiditis complicated with thyroid cancer than in patients with simple Hashimoto thyroiditis. BRAF600E mutation may be a high risk factor for Hashimoto thyroiditis complicated with thyroid cancer. So as to provide help for early diagnosis of thyroid cancer.
【作者单位】：山东临沂市沂水中心医院;
【分类号】：R736.1;R581.4

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