小麦凝集素激酶TaLecRK-Ⅳ.1基因的作用和由纹枯病诱导的小麦基因发展的分子标记
发布时间：2020-12-29 20:28
小麦纹枯病主要由土传真菌Rhizoctonia cerealis侵染小麦茎基部引起。它已经成为世界范围内小麦生产的限制性因素,在中国更为严重。小麦纹枯病的防治已经成为小麦生产可持续发展的主要问题。培育和种植抗性小麦品种是防治小麦纹枯病最环保、高效的方法。小麦纹枯病抗性遗传机制研究有助于解析小麦抗性相关基因的功能和作用。本研究中,我们从抗纹枯病的小麦材料CI12633中鉴定了一个L型凝集素受体激酶基因,命名为TaLecRK-Ⅳ.1。与感纹枯病小麦温麦6号相比,在CI12633中该基因的表达受纹枯病侵染诱导。序列分析表明,该基因编码含有676个氨基酸残基的L型凝集素受体激酶。利用病毒诱导基因沉默技术（virus-induced gene silencing,VIGS）技术沉默CI12633的TaLecRK-Ⅳ.1后,小麦植株表现矮化的表型,表明该基因可能是小麦矮化的负调控因子。进一步在含有矮化基因Rht-D1b（formerly Rht2）的小麦中分析了该基因的转录,结果表面TaLecRK-Ⅳ.1在矮化小麦中的表达量很低。另外,参与植物生长和发育的激素---赤霉素和生长素诱导TaLecRK...
【文章来源】：中国农业科学院北京市
【文章页数】：118 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
Chapter 1 Introduction and literature review
1.1 Introduction
1.1.1 History and origin of wheat
1.1.2 Social and economic importance
1.1.3 Wheat cultivation around the world
1.1.4 Influence of plant height on wheat production
1.1.5 Role of phytohormones on plant growth
1.1.6 Factor that favor wheat adaptability
1.1.7 Constraints of wheat production
1.1.8 Wheat sharp eyespot
1.1.9 Employment of RNA-seq to identify putative sharp eyespot resistant genes
1.1.10 Gene introgression from wheat wild relatives
1.1.11 Importance of molecular markers in crop breeding
1.1.12 Molecular marker development
1.1.13 Recombinant inbred line （RIL） populations in breeding research
1.2 Literature review
1.2.1 Sharp eyespot
1.2.2 Plants and disease
1.2.3 Plant defense mechanisms
1.2.4 Signal perception
1.2.5 Genes mediated resistances
1.2.6 Hypersensitive response （HR）
1.2.7 Biochemical defenses
1.2.8 Pathogenesis-related proteins
1.3 Purpose of the study
Chapter 2 Silencing of L-type lectin-like receptor kinase gene Ta Lec RK-Ⅳ.1 leads to dwarf phenotype in wheat line CI12633
Introduction
2.1 Materials and Methods
2.1.1 Plant and fungal materials, growth conditions and treatments
2.1.2 RNA Sequencing-based transcriptome analysis
2.1.3 Methodology
2.1.4 Virus-Induced Gene Silencing
2.1.5 BSMV inoculation
2.1.6 Test for susceptibility to sharp eyespot
2.1.7 Phytohormones treatment （GA, Auxin, ABA and SA）
2.2 Results
2.2.1 Identification of Ta Lc RK-Ⅳ.1 by RNA-Seq and cloning of the gene sequence
2.2.2 Ta Lc RK encodes an L-type lectin domain containing receptor kinase Ⅳ.1
2.2.3 Chromosomal location of Ta Lec RK -Ⅳ.1
2.2.4 Phenotype resulting from the gene silencing experiment
2.2.5 Sharp eyespot responses of the plants subjected to the VIGS experiment
2.2.6 Relative expression of Ta Lec RK-Ⅳ.1 and its homologue gene in wheat line with Rht-D1b
2.2.7 Analysis of the expression of GA biosynthetic enzymes Ta GA20ox and Ta GA3ox2 in Rht2 wheat line and CI12633 plants inoculated with BSMV:Ta Lec RK-Ⅳ.1
2.2.8 Expression patterns of Ta Lec RK-Ⅳ.1 in response to exogenous hormone treatments
Discussion
Chapter 3 Analysis for quantitative loci using molecular markers derived from wheat genes induced by sharp eyespot
Introduction
3.1 Materials and methods
3.1.1 Plant materials
3.1.2 Pathogen material
3.1.3 Pathogen tests
3.1.4 Pathogen assessment
3.1.5 DNA isolation and PCR amplification
3.1.6 Linkage map construction and QTL analysis
3.2 Results
3.2.1 Phenotypic assessments of sharp eyespot infection
3.2.2 Marker development, genotyping and linkage map construction
3.2.3 Analysis of the phenotypic and genotypic data for QTL associated with the resistance trait
Discussion
Chapter 4 General discussion
Summary
References
Acknowledgements
Funding
Manuscript in preparation
【参考文献】：
期刊论文
[1]小麦抗纹枯病种质资源筛选[J]. 任丽娟,陈佩度,陈怀谷,马鸿翔. 植物遗传资源学报. 2010(01)
[2]小麦抗纹枯病种质创新及QTL定位的初步研究[J]. 蔡士宾,任丽娟,颜伟,吴纪中,陈怀谷,吴小有,张仙义. 中国农业科学. 2006(05)
[3]小麦纹枯病抗性的QTL分析和抗病基因的分子标记[J]. 张小村,李斯深,赵新华,范玉顶,李瑞军. 植物遗传资源学报. 2005(03)
[4]小麦纹枯病抗性QTL的SSR标记研究[J]. 任丽娟,蔡士宾,汤頲,吴纪中,周淼平,颜伟,马鸿翔,陆维忠. 扬州大学学报. 2004(04)
[5]江苏省小麦纹枯病发生规律与病害消长因素分析[J]. 王裕中,吴志凤,史建荣,陈怀谷,邵伯坤. 植物保护学报. 1994(02)
本文编号：2946265
【文章来源】：中国农业科学院北京市
【文章页数】：118 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
Chapter 1 Introduction and literature review
1.1 Introduction
1.1.1 History and origin of wheat
1.1.2 Social and economic importance
1.1.3 Wheat cultivation around the world
1.1.4 Influence of plant height on wheat production
1.1.5 Role of phytohormones on plant growth
1.1.6 Factor that favor wheat adaptability
1.1.7 Constraints of wheat production
1.1.8 Wheat sharp eyespot
1.1.9 Employment of RNA-seq to identify putative sharp eyespot resistant genes
1.1.10 Gene introgression from wheat wild relatives
1.1.11 Importance of molecular markers in crop breeding
1.1.12 Molecular marker development
1.1.13 Recombinant inbred line （RIL） populations in breeding research
1.2 Literature review
1.2.1 Sharp eyespot
1.2.2 Plants and disease
1.2.3 Plant defense mechanisms
1.2.4 Signal perception
1.2.5 Genes mediated resistances
1.2.6 Hypersensitive response （HR）
1.2.7 Biochemical defenses
1.2.8 Pathogenesis-related proteins
1.3 Purpose of the study
Chapter 2 Silencing of L-type lectin-like receptor kinase gene Ta Lec RK-Ⅳ.1 leads to dwarf phenotype in wheat line CI12633
Introduction
2.1 Materials and Methods
2.1.1 Plant and fungal materials, growth conditions and treatments
2.1.2 RNA Sequencing-based transcriptome analysis
2.1.3 Methodology
2.1.4 Virus-Induced Gene Silencing
2.1.5 BSMV inoculation
2.1.6 Test for susceptibility to sharp eyespot
2.1.7 Phytohormones treatment （GA, Auxin, ABA and SA）
2.2 Results
2.2.1 Identification of Ta Lc RK-Ⅳ.1 by RNA-Seq and cloning of the gene sequence
2.2.2 Ta Lc RK encodes an L-type lectin domain containing receptor kinase Ⅳ.1
2.2.3 Chromosomal location of Ta Lec RK -Ⅳ.1
2.2.4 Phenotype resulting from the gene silencing experiment
2.2.5 Sharp eyespot responses of the plants subjected to the VIGS experiment
2.2.6 Relative expression of Ta Lec RK-Ⅳ.1 and its homologue gene in wheat line with Rht-D1b
2.2.7 Analysis of the expression of GA biosynthetic enzymes Ta GA20ox and Ta GA3ox2 in Rht2 wheat line and CI12633 plants inoculated with BSMV:Ta Lec RK-Ⅳ.1
2.2.8 Expression patterns of Ta Lec RK-Ⅳ.1 in response to exogenous hormone treatments
Discussion
Chapter 3 Analysis for quantitative loci using molecular markers derived from wheat genes induced by sharp eyespot
Introduction
3.1 Materials and methods
3.1.1 Plant materials
3.1.2 Pathogen material
3.1.3 Pathogen tests
3.1.4 Pathogen assessment
3.1.5 DNA isolation and PCR amplification
3.1.6 Linkage map construction and QTL analysis
3.2 Results
3.2.1 Phenotypic assessments of sharp eyespot infection
3.2.2 Marker development, genotyping and linkage map construction
3.2.3 Analysis of the phenotypic and genotypic data for QTL associated with the resistance trait
Discussion
Chapter 4 General discussion
Summary
References
Acknowledgements
Funding
Manuscript in preparation
【参考文献】：
期刊论文
[1]小麦抗纹枯病种质资源筛选[J]. 任丽娟,陈佩度,陈怀谷,马鸿翔. 植物遗传资源学报. 2010(01)
[2]小麦抗纹枯病种质创新及QTL定位的初步研究[J]. 蔡士宾,任丽娟,颜伟,吴纪中,陈怀谷,吴小有,张仙义. 中国农业科学. 2006(05)
[3]小麦纹枯病抗性的QTL分析和抗病基因的分子标记[J]. 张小村,李斯深,赵新华,范玉顶,李瑞军. 植物遗传资源学报. 2005(03)
[4]小麦纹枯病抗性QTL的SSR标记研究[J]. 任丽娟,蔡士宾,汤頲,吴纪中,周淼平,颜伟,马鸿翔,陆维忠. 扬州大学学报. 2004(04)
[5]江苏省小麦纹枯病发生规律与病害消长因素分析[J]. 王裕中,吴志凤,史建荣,陈怀谷,邵伯坤. 植物保护学报. 1994(02)
本文编号：2946265
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