苏云金芽胞杆菌MEP代谢途径相关基因的转录调控研究
发布时间：2021-01-12 17:44
苏云金芽胞杆菌（Bacillus thuringensis,Bt）,是一种革兰氏阳性细菌,在生成芽胞的同时还产生对多种农林害虫有杀虫活性的伴胞晶体,是应用最广的微生物杀虫剂。萜类化合物广泛分布在生物界,是重要的生命物质。目前发现有两条萜类化合物的生物合成途径,即甲羟戊酸（MVA）途径和2-甲基-D-赤藓糖醇-4-磷酸（MEP）途径。Bt中有8个基因编码MEP代谢途径相关的酶,本文对这些基因的转录调控进行研究。主要取得了如下结果:生物信息学分析发现,Bt中有8个基因编码MEP代谢途径相关的酶,它们是:dxs基因（HD734479）编码1-脱氧-D-木酮糖-5-磷酸合酶,dxr基因（HD733605/dxr1和HD734104/dxr2）编码1-脱氧-D-木酮糖-5-磷酸还原异构酶,ispD（HD730085）基因编码2-C-甲基-D-赤藓醇-4-磷酸胞苷转移酶,ispE（HD730043）基因编码4-胞啶-2-C-甲基-D-赤藓醇激酶,ispF（HD7300...
【文章来源】：山西师范大学山西省
【文章页数】：93 页
【学位级别】：硕士
【部分图文】：
比较苏云金芽胞杆菌HD73与枯草芽孢杆菌168菌株MEP途径Fig.3-1ComparisonofMEPpathwaybetweeninBtHD73andBs168stain对两个菌株的MEP代谢途径相关基因进行ProteinBlast比对（表3-1）
结果与分析31表3-1苏云金芽胞杆菌HD73菌株MEP代谢途径生物信息学分析Table3-1BioinformaticsanalysisofMEPpathwayinBtHD73strainGeneIDGenenameBs168IdentityGeneannotationsHD73_4479dxsBSU2427077.8%1-deoxy-D-xylulose-5-phosphatesynthaseHD73_3605dxrBSU1655056.5%1-deoxy-D-xylulose-5-phosphatereductoisomeraseHD73_4104dxrBSU1655070.1%1-deoxy-D-xylulose5-phosphatereductoisomeraseHD73_0085ispDBSU0090053.4%2-C-methyl-D-erythritol4-phosphatecytidylyltransferaseHD73_0043ipk/ispEBSU0046074.0%4-diphosphocytidyl-2-C-methyl-D-erythritolkinaseHD73_0086ispFBSU0091076.4%2-C-methyl-D-erythritol2,4-cyclodiphosphatesynthaseHD73_4584ispGBSU2507089.0%4-hydroxy-3-methylbut-2-en-1-yldiphosphatesynthaseHD73_4593ispHBSU2516074.1%4-hydroxy-3-methylbut-2-enyldiphosphatereductaseHD73_1728fniBSU2287060.2%isopentenyl-diphosphatedelta-isomeraseHD73_4480ispABSU2428059.5%farnesyldiphosphatesynthaseHD73_1741hepSBSU2276040.0%heptaprenyldiphosphatesynthasecomponentIHD73_1743hepTBSU2274063.8%heptaprenyldiphosphatesynthasecomponentIIHD73_4106uppSBSU1653070.5%undecaprenylpyrophosphatesynthase图3-2dxs与ispA基因在BtHD73和Bs168中分布比较Fig.3-2ComparisonofdxsandispAgenebetweeninBtHD73andBs168stain
山西师范大学硕士学位论文32图3-3dxr基因在BtHD73和Bs168中分布比较Fig.3-3ComparisonoftwodxrgenesbetweeninBtHD73andBs168stain图3-4ispD与ispF基因在BtHD73和Bs168中分布比较Fig.3-4ComparisonofispDandispFgenebetweeninBtHD73andBs168stain图3-5ispE基因在BtHD73和Bs168中分布比较Fig.3-5ComparisonofispEgenebetweeninBtHD73andBs168stain图3-6ispG基因在BtHD73和Bs168中分布比较Fig.3-6ComparisonofispGgenebetweeninBtHD73andBs168stain
本文编号：2973245
【文章来源】：山西师范大学山西省
【文章页数】：93 页
【学位级别】：硕士
【部分图文】：
比较苏云金芽胞杆菌HD73与枯草芽孢杆菌168菌株MEP途径Fig.3-1ComparisonofMEPpathwaybetweeninBtHD73andBs168stain对两个菌株的MEP代谢途径相关基因进行ProteinBlast比对（表3-1）
结果与分析31表3-1苏云金芽胞杆菌HD73菌株MEP代谢途径生物信息学分析Table3-1BioinformaticsanalysisofMEPpathwayinBtHD73strainGeneIDGenenameBs168IdentityGeneannotationsHD73_4479dxsBSU2427077.8%1-deoxy-D-xylulose-5-phosphatesynthaseHD73_3605dxrBSU1655056.5%1-deoxy-D-xylulose-5-phosphatereductoisomeraseHD73_4104dxrBSU1655070.1%1-deoxy-D-xylulose5-phosphatereductoisomeraseHD73_0085ispDBSU0090053.4%2-C-methyl-D-erythritol4-phosphatecytidylyltransferaseHD73_0043ipk/ispEBSU0046074.0%4-diphosphocytidyl-2-C-methyl-D-erythritolkinaseHD73_0086ispFBSU0091076.4%2-C-methyl-D-erythritol2,4-cyclodiphosphatesynthaseHD73_4584ispGBSU2507089.0%4-hydroxy-3-methylbut-2-en-1-yldiphosphatesynthaseHD73_4593ispHBSU2516074.1%4-hydroxy-3-methylbut-2-enyldiphosphatereductaseHD73_1728fniBSU2287060.2%isopentenyl-diphosphatedelta-isomeraseHD73_4480ispABSU2428059.5%farnesyldiphosphatesynthaseHD73_1741hepSBSU2276040.0%heptaprenyldiphosphatesynthasecomponentIHD73_1743hepTBSU2274063.8%heptaprenyldiphosphatesynthasecomponentIIHD73_4106uppSBSU1653070.5%undecaprenylpyrophosphatesynthase图3-2dxs与ispA基因在BtHD73和Bs168中分布比较Fig.3-2ComparisonofdxsandispAgenebetweeninBtHD73andBs168stain
山西师范大学硕士学位论文32图3-3dxr基因在BtHD73和Bs168中分布比较Fig.3-3ComparisonoftwodxrgenesbetweeninBtHD73andBs168stain图3-4ispD与ispF基因在BtHD73和Bs168中分布比较Fig.3-4ComparisonofispDandispFgenebetweeninBtHD73andBs168stain图3-5ispE基因在BtHD73和Bs168中分布比较Fig.3-5ComparisonofispEgenebetweeninBtHD73andBs168stain图3-6ispG基因在BtHD73和Bs168中分布比较Fig.3-6ComparisonofispGgenebetweeninBtHD73andBs168stain
本文编号：2973245
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