渗透胁迫下sakA/hogA基因对黄曲霉毒素合成及致病性的影响
发布时间：2021-01-15 09:13
黄曲霉是腐生真菌,属于曲霉属。这种真菌是一种机会致病菌,能够污染大多数种子作物和其他生物,包括动物和人类。更严重的是,黄曲霉由于其次级代谢产物的高毒性而导致死亡或慢性疾病。因此,影响黄曲霉生长和分布的各种因素、黄曲霉毒素生物合成途径、基因簇以及调控机制已成为过去几十年研究的主题,其中包括响应于渗透压的（MAPK/HOG）途径促分裂原活化蛋白激酶信号的研究。尽管该途径已在一些真菌中广泛研究,但在黄曲霉中,其机制尚不清楚。Sak A（hog1/hogA）被认为是MAPK/HOG途径的重要部分,为此本研究重点是确定Sak A在黄曲霉中的生物学功能。在本研究中,通过同源重组的方法进行敲除和互补菌株的构建,随后通过1.2mo L/L的氯化钠（NaCl）、1.2 mo L/L D-山梨醇、以及0.93和0.99水活度诱导渗透压。实验发现,与野生型（WT）和互补菌株（ΔAfsakA::AfsakA）相比,基因敲除株（ΔAfsakA）对分生孢子产生显著影响。q RT-PCR结果显示,aba A和brl A基因的表达水平在ΔAfsakA中,比WT和ΔAfsakA::AfsakA菌株中发生下调。研究还发现...
【文章来源】：福建农林大学福建省
【文章页数】：123 页
【学位级别】：硕士
【部分图文】：
黄曲霉毒素侵入人体循环和致病的图解
硕士学位论文 Osmotic-adaptation response of sakA/hogA gene to aflatoxin biosynthesis, morphologydevelopment and pathogenicity in Aspergillus flavus
硕士学位论文 Osmotic-adaptation response of sakA/hogA gene to aflatoxin biosynthesis, morphologydevelopment and pathogenicity in Aspergillus flavusMAPKs in Saccharomyces cerevisiae is conserved with GTP-binding proteins, protein kinases,adaptor, scaffold, activating proteins, cell surface proteins, activators, inhibitors and enzymesconnecting the surface of the cell to intracellular regulatory targets activating differentmorphogenetic changes[109, 123]. Saccharomyces cerevisiae has five MAPKs pathways (Figure 1-4) which consist of protein kinases responsible for fungal regulator namely FUS3, KSS1, HOG1,SLT2, SMK1 and they also identify processes and categorize them in different groups likepheromone response, filamentous growth, osmoadaptation, cell wall integrity, and ascosporeformation respectively[123].
本文编号：2978638
【文章来源】：福建农林大学福建省
【文章页数】：123 页
【学位级别】：硕士
【部分图文】：
黄曲霉毒素侵入人体循环和致病的图解
硕士学位论文 Osmotic-adaptation response of sakA/hogA gene to aflatoxin biosynthesis, morphologydevelopment and pathogenicity in Aspergillus flavus
硕士学位论文 Osmotic-adaptation response of sakA/hogA gene to aflatoxin biosynthesis, morphologydevelopment and pathogenicity in Aspergillus flavusMAPKs in Saccharomyces cerevisiae is conserved with GTP-binding proteins, protein kinases,adaptor, scaffold, activating proteins, cell surface proteins, activators, inhibitors and enzymesconnecting the surface of the cell to intracellular regulatory targets activating differentmorphogenetic changes[109, 123]. Saccharomyces cerevisiae has five MAPKs pathways (Figure 1-4) which consist of protein kinases responsible for fungal regulator namely FUS3, KSS1, HOG1,SLT2, SMK1 and they also identify processes and categorize them in different groups likepheromone response, filamentous growth, osmoadaptation, cell wall integrity, and ascosporeformation respectively[123].
本文编号：2978638
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