卡波西肉瘤病毒编码的kshv-miR-K12-1-5p靶基因预测及生物信息学分析
发布时间：2021-03-11 14:50
目的:对卡波西肉瘤病毒（Kaposi’s sarcoma-associated herpesvirus,KSHV）编码的小分子RNA kshv-miR-K12-1-5p进行靶基因预测和靶基因生物信息学分析,为未来对kshv-miR-K12-1-5p靶基因的研究提供可行的方向。方法:利用数据库PubMed、miRTarBase、TargetScanHuman Custom、miRDB等对kshv-miR-K12-1-5p靶基因进行查询和预测,之后对这些靶基因进行GO和KEGG的富集分析以及蛋白互作网络的分析,并对互作网络的关键模块和关键基因进行提取和分析。结果:通过数据库miRTarBase查询到经过验证的靶基因有NF-Kappa-B抑制因子α和细胞转录因子MAF;通过PubMed查询到经过验证的靶基因有抑制细胞因子信号转导6。通过生物信息学分析预测得到8个靶基因,分别是PPIH、POLR2B、HNRNPU、MAGOHB、DNAJC8、HNRNPC、RBM22、HNRNPA3。结论:本次研究通过利用生物信息学的方法,对KSHV编码的kshv-miR-K12-1-5p靶基因进行预测,得到一...
【文章来源】：肿瘤预防与治疗. 2020,33(12)
【文章页数】：7 页
【部分图文】：
TargetScan Human Custom和miRDB预测得到的kshv-miR-K12-1-5p靶基因
表2 关键基因的简要介绍Table 2. Brief Introduction of Hubgenes No. Name Full name Brief introduction 1 PPIH Peptidylprolyl isomerase H Diseases associated with PPIH include tropical sprue and urofacial syndrome 1 2 POLR2B RNA polymerase II subunit B Diseases associated with POLR2B include Fusariosis and eumycotic mycetoma 3 HNRNPU Heterogeneous nuclear ribonucleoprotein U Diseases associated with HNRNPU include epileptic encephalopathy, early infantile, 54 and visual epilepsy 4 MAGOHB Mago homolog B, exon junction complex subunit Among its related pathways are cleavage of growing transcript in the termination region and transport of mature transcript to cytoplasm 5 DNAJC8 DnaJ heat shock protein family (Hsp40) member C8 A protein coding gene; among its related pathways are gene expression and mRNA splicing-major pathway 6 HNRNPC Heterogeneous nuclear ribonucleoprotein C Diseases associated with HNRNPC include mixed connective tissue disease and oculopharyngeal muscular dystrophy 7 RBM22 RNA binding motif protein 22 Required for pre-mRNA splicing as component of the activated spliceosome, involved in the first step of pre-mRNA splicing 8 HNRNPA3 Heterogeneous nuclear ribonucleoprotein A3 Diseases associated with HNRNPA3 include dermatopathia pigmentosa reticularis and multisystem proteinopathy图3 使用Cytoscape 软件MCODE插件获取的靶基因网络中最关键模块
图2 STRING数据库获取的kshv-miR-K12-1-5p靶基因蛋白互作网络图4 使用Cytoscape 软件 BiNGO 插件获得的最关键基因的富集结果
本文编号：3076639
【文章来源】：肿瘤预防与治疗. 2020,33(12)
【文章页数】：7 页
【部分图文】：
TargetScan Human Custom和miRDB预测得到的kshv-miR-K12-1-5p靶基因
表2 关键基因的简要介绍Table 2. Brief Introduction of Hubgenes No. Name Full name Brief introduction 1 PPIH Peptidylprolyl isomerase H Diseases associated with PPIH include tropical sprue and urofacial syndrome 1 2 POLR2B RNA polymerase II subunit B Diseases associated with POLR2B include Fusariosis and eumycotic mycetoma 3 HNRNPU Heterogeneous nuclear ribonucleoprotein U Diseases associated with HNRNPU include epileptic encephalopathy, early infantile, 54 and visual epilepsy 4 MAGOHB Mago homolog B, exon junction complex subunit Among its related pathways are cleavage of growing transcript in the termination region and transport of mature transcript to cytoplasm 5 DNAJC8 DnaJ heat shock protein family (Hsp40) member C8 A protein coding gene; among its related pathways are gene expression and mRNA splicing-major pathway 6 HNRNPC Heterogeneous nuclear ribonucleoprotein C Diseases associated with HNRNPC include mixed connective tissue disease and oculopharyngeal muscular dystrophy 7 RBM22 RNA binding motif protein 22 Required for pre-mRNA splicing as component of the activated spliceosome, involved in the first step of pre-mRNA splicing 8 HNRNPA3 Heterogeneous nuclear ribonucleoprotein A3 Diseases associated with HNRNPA3 include dermatopathia pigmentosa reticularis and multisystem proteinopathy图3 使用Cytoscape 软件MCODE插件获取的靶基因网络中最关键模块
图2 STRING数据库获取的kshv-miR-K12-1-5p靶基因蛋白互作网络图4 使用Cytoscape 软件 BiNGO 插件获得的最关键基因的富集结果
本文编号：3076639
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/3076639.html
最近更新
教材专著
