玉米籽粒镉低积累基因型的筛选与相关机理的研究

发布时间：2021-09-04 14:10

　　土壤镉污染严重影响作物的产量和品质,并通过食物链威胁人类健康。对于受轻度或中度镉污染的土壤,培育和种植耐镉且食用器官低积累作物基因型/品种,是有效利用自然资源和保证农产品安全生产的重要途径之一。玉米（Zea mays L.）是目前世界上产量最高的粮食作物。本研究在分析比较95个玉米基因型（自交系）籽粒镉含量筛选获得籽粒镉积累不同玉米基因型的基础上,分析了镉胁迫对不同籽粒镉积累基因型玉米生长、植物激素等的影响及基因型差异,并通过比较转录组分析,探讨了镉耐性与低积累的相关基因。主要研究结果如下:1.玉米籽粒镉低积累基因型筛选分析评估95份玉米基因型（自交系）籽粒镉含量,筛选籽粒镉低积累基因型。连续两年的田间试验在浙江大学长兴农业试验站进行,分析测定籽粒镉含量,结果表明,相同栽培条件下,95个玉米基因型籽粒镉含量平均为0.013 mg kg^-1 DW,变化范围为0.001-0.06 mg Cd kg^-1DW,基因型间差异显著。连续两年的试验结果显示,籽粒镉含量基因型差异的变化趋势完全一致,其中籽粒镉含量最低和最高的分别为:基因型L42、L15和L5... 

【文章来源】：浙江大学浙江省 211工程院校 985工程院校 教育部直属院校

【文章页数】：78 页

【学位级别】：硕士

【文章目录】：
Acknowledgements
List of Abbreviations
ABSTRACT
摘要
CHAPTER1:Introduction
CHAPTER2:Literature review
    2.1 Cd toxicity
    2.2 Approaches for decreasing grain Cd accumulation
    2.3 Cadmium uptake,translocation and accumulation in grains
    2.4 Advances in molecular mechanisms of Cd transport and tolerance
    2.5 Research Objectives
CHAPTER3 Identification of maize genotypes with low grain Cd accumulation
    3.1 Material and Methods
        3.1.1 Field experiments design
        3.1.2 Analysis of heavy metal concentration in grains
        3.1.3 Statistical analysis
    3.2 Results
        3.2.1 Heavy metal concentrations in maize grains
        3.2.2 Correlation analysis among grain heavy metal concentrations
    3.3 Discussion
CHAPTER4 Differences in physiological features in response to Cd stress in the two maize genotypes with contrasting grain Cd accumulation
    4.1 Materials and Methods
        4.1.1 Plant materials and growth conditions
        4.1.2 Determination of heavy metal contents
        4.1.3 Measurement of plant growth parameters
        4.1.4 TEM(transmission electron microscope)ultrastructure
        4.1.5 Phytohormones determination
        4.1.6 Statistical analysis
    4.2 Results
        4.2.1 Plant growth parameters
        4.2.2 Cd content
        4.2.3 Element Analysis
        4.2.4 Phytohormone contents
        4.2.5 Examination of root ultrastructure
        4.2.6 Examination of leaf ultrastructure
    4.3 Discussion
CHAPTER5:Comparative transcription study in response to Cd stress between the two maize genotypes with contrasting grain Cd accumulation
    5.1 Materials and Methods
        5.1.1 Plant materials and growth conditions
        5.1.2 RNA isolation
        5.1.3 Library preparation and sequencing of RNAs
        5.1.4 Genome mapping
        5.1.5 qRT-PCR validation
    5.2 Results
        5.2.1 Functional categorization
        5.2.2 qRT-PCR confirms expression pattern of the transcriptome data
    5.3 Discussion
CHAPTER6 Major findings and future prospects
    6.1 Major Findings
    6.2 Future Prospects
References
Supplementary



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