血清抵抗素水平及抵抗素基因启动子-420C/G多态性与川崎病的相关性研究
发布时间：2021-11-24 23:00
目的观察血清中抵抗素水平及抵抗素基因启动子-420C/G多态性在川崎病的表达水平,并对其相关性进行分析。方法随机选择60例川崎病（KD）患儿为KD组,另设60例健康儿童为对照组。两组均采用酶联免疫吸附实验检测儿童血清抵抗素水平,应用多聚酶链反应限制性片段长度多态性技术检测不同组别患儿抵抗素基因-420C/G位点多态性的差异,同时检测统计其血液学相关指标,包括血白细胞计数（WBC）、红细胞计数（RBC）、血红蛋白水平（Hb）,红细胞压积（Hct）。结果与对照组比较,KD患儿外周血液中WBC、RBC、Hb及Hct表达水平均降低（P<0.05）,而血小板计数（PLT）表达水平升高（P<0.05）,差异有统计学意义;血清抵抗素检测结果显示,与对照组相比,KD患儿血清抵抗素水平升高;限制性核酸内切酶试剂盒BpiⅠ检测血清抵抗素基因启动子-420C/G多态性,与对照组相比,KD组患儿GG较CC基因频率较低（P<0.05）。Pearson相关分析显示,KD患儿血浆抵抗素水平与WBC、RBC、Hb及Hct呈负相关（P<0.05）,而与PLT表达水平呈正相关（P<0.05）...
【文章来源】：中国现代医学杂志. 2017,27(20)北大核心
【文章页数】：5 页
【文章目录】：
1 资料与方法
1.1 研究对象
1.2 试剂及仪器
1.3 血液学指标检测
1.4 实验方法
1.5 统计学方法
2 结果
2.1 一般血液学指标比较
2.2 血清抵抗素水平比较
2.3 血清抵抗素基因启动子-420C/G多态性
2.4 KD组血浆中抵抗素水平与其他指标的相关性分析
2.5 不同基因型患儿的生化指标比较
3 讨论
【参考文献】：
期刊论文
[1]川崎病患儿治疗前后血清抵抗素和内脂素的变化[J]. 付淑芳,俞大亮,吕典一,陈凤仪. 中国当代儿科杂志. 2014(01)
[2]High Serum Resistin Level may be an Indicator of the Severity of Coronary Disease in Acute Coronary Syndrome[J]. Hao Wang1, De-you Chen2, Jian Cao1, Zuo-yun He3, Bing-po Zhu1, and Min Long41Department of Geriatric Cardiology, 2Out-patient Department, Chinese People’s Liberation Army General Hospital, Beijing 100853, China 3Department of Internal Cardiology, 4Department of Endocrinology, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China. Chinese Medical Sciences Journal. 2009(03)
[3]Plasma resistin is increased in patients with unstable angina[J]. HU Wen-lan QIAO Shu-bin HOU Qing YUAN Jian-song Department of Cardiology, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China (Hu WL Qiao SB Hou Q Yuan JS). Chinese Medical Journal. 2007(10)
[4]Role of Resistin in Inflammation and Inflammation-Related Diseases[J]. Shanshan Pang and Yingying Le Laboratory of Immunologic and Inflammatory Diseases, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences; Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China. Cellular & Molecular Immunology. 2006(01)
本文编号：3516917
【文章来源】：中国现代医学杂志. 2017,27(20)北大核心
【文章页数】：5 页
【文章目录】：
1 资料与方法
1.1 研究对象
1.2 试剂及仪器
1.3 血液学指标检测
1.4 实验方法
1.5 统计学方法
2 结果
2.1 一般血液学指标比较
2.2 血清抵抗素水平比较
2.3 血清抵抗素基因启动子-420C/G多态性
2.4 KD组血浆中抵抗素水平与其他指标的相关性分析
2.5 不同基因型患儿的生化指标比较
3 讨论
【参考文献】：
期刊论文
[1]川崎病患儿治疗前后血清抵抗素和内脂素的变化[J]. 付淑芳,俞大亮,吕典一,陈凤仪. 中国当代儿科杂志. 2014(01)
[2]High Serum Resistin Level may be an Indicator of the Severity of Coronary Disease in Acute Coronary Syndrome[J]. Hao Wang1, De-you Chen2, Jian Cao1, Zuo-yun He3, Bing-po Zhu1, and Min Long41Department of Geriatric Cardiology, 2Out-patient Department, Chinese People’s Liberation Army General Hospital, Beijing 100853, China 3Department of Internal Cardiology, 4Department of Endocrinology, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China. Chinese Medical Sciences Journal. 2009(03)
[3]Plasma resistin is increased in patients with unstable angina[J]. HU Wen-lan QIAO Shu-bin HOU Qing YUAN Jian-song Department of Cardiology, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China (Hu WL Qiao SB Hou Q Yuan JS). Chinese Medical Journal. 2007(10)
[4]Role of Resistin in Inflammation and Inflammation-Related Diseases[J]. Shanshan Pang and Yingying Le Laboratory of Immunologic and Inflammatory Diseases, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences; Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China. Cellular & Molecular Immunology. 2006(01)
本文编号：3516917
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