miR-1827抑制甲状腺乳头状癌细胞增殖、侵袭转移及对c-Myc基因表达的影响（英文）

发布时间：2023-05-06 05:11

　　目的:探讨微小RNA 1827(mi R-1827)对甲状腺乳头状癌细胞增殖、侵袭转移及对c-Myc基因表达的影响。方法:采用实时荧光定量聚合酶链反应(q PCR)检测mi R-1827在TPC-1和Nthy-ori 3-1细胞中的表达,分别用CCK-8、细胞侵袭和蛋白质印迹法(Western blot)检测过表达及干扰mi R-1827对TPC-1细胞增殖、侵袭和上皮间质转化(EMT)的影响。采用Target Scan和双荧光素酶报告基因实验分析mi R-1827与c-Myc之间的作用靶点,通过q PCR和Western blot检测mi R-1827和c-Myc之间的调控关系。通过Western blot分析mi R-1827对TPC-1细胞中Akt-m TOR通路的影响。雷帕霉素抑制Akt-m TOR通路后,检测mi R-1827对甲状腺乳头状癌细胞增殖、侵袭转移以及对c-Myc基因表达的影响。结果:TPC-1细胞中mi R-1827表达水平明显低于Nthy-ori 3-1细胞(P<0.01),过表达mi R-1827能够抑制TPC-1细胞的增殖和侵袭(P<0.05)...

【文章页数】：9 页

【文章目录】：
Introduction
Materials and methods
    Main materials
Methods
    Cell culture
    Cell transfection
    Cell proliferation assay
    Detection of dual luciferase reporter gene activity
    Cell invasion assay
    RT-q PCR
    Western blot
    Statistical method
Results
    Overexpression of mi R-1827 inhibited TPC-1 cell proliferation,invasion and epithelial-mesenchymal transition(EMT)
    Down-regulation of mi R-1827 promotes proliferation,invasion and EMT of TPC-1 cell
    Mi R-1827 regulates proliferation,invasion and EMT of TPC-1 cell through Akt-m TOR pathway
    Targeting and regulating relationship between mi R-1827 and c-Myc
    Mi R-1827 regulates c-Myc expression through Akt-m TOR pathway
Conclusion



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